桑仙降压颗粒对自发性高血压大鼠心肌MVD和ESM1蛋白表达的干预

The Intervention of MVD and ESM1 Protein Expression of Sangxianjiangya Granule on Myocardium of Spontaneously Hypertensive rats

目的观察桑仙降压颗粒对自发性高血压大鼠(SHR)心肌微血管密度(MVD)及内皮细胞特异性分子-1(ESM1)蛋白的影响,探讨其对高血压心肌微血管数目的干预及作用机制。方法 SHR随机分为空白对照组、吲哒帕胺西药对照组、桑仙降压颗粒组,以Wistar大鼠为正常对照,4周及8周后观察SHR心肌MVD及ESM1蛋白的表达。结果相较空白对照组,实验4周桑仙降压颗粒组、吲哒帕胺组CD34表达仅有增多趋势,实验8周时两组CD34表达明显增多,实验4周、8周,与空白对照组相比,桑仙降压颗粒组、吲哒帕胺西药对照组ESM1蛋白表达均增高,均有显著性差异。结论桑仙降压颗粒能有效预防SHR心脏微血管减少,其机制之一可能与上调ESM1蛋白表达有关。

Objective： To observe the Sangxianjiangya Granule（SXJYG） on blood pressure in spontaneously hypertensive rats（SHR） myocardial microvascular density（MVD） and endothelial cell specific molecule-l（ESM1） protein effects,and to investigate its effect on hypertensive myocardial microvascular number of intervention and mechanism.Methods： SHR were randomly divided into control group,indapamide western medicine group,SXJYG group,in Wister rats as normal control,SHR of myocardial MVD and ESM1 protein expression were observed after 4 weeks and 8 weeks.Results： compared with the control group,the experimental group 4 weeks,SXJYG group and indapamide group CD34 expression only increased trendly after 4 weeks and increased markedly after 8 weeks.Compared with blank group,SXJYG group and indapamide western medicine control group ESM1 protein expression were increased after 4 weeks and 8 weeks,and there were significant differences.Conclusion： SXJYG is effective in the prevention of SHR cardiac microvessel reduction,and one of its mechanisms may be associated with upregulation of ESM1 protein expression.