穿心莲药材质量的化学和药效学评价相关性研究

Correlative Studies on Chemistry and Pharmacodynamics Estimation of Quality of Andrographis paniculata(Burm.f.) Nees

目的:为中药质量药理学评价方法体系的建立提供正面依据。方法:采用高效液相检测五个批次穿心莲药材中穿心莲内酯和脱水穿心莲内酯的含量;采用二甲苯所致小鼠耳肿胀试验表征药材的抗炎作用;采用细菌脂多糖所致大鼠发热试验表征药材的解热作用;根据上述结果分析用化学和药效学方法评估穿心莲药材质量的相关性。结果:不同批次药材的穿心莲内酯和脱水穿心莲内酯含量存在差异,其总含量分别为3.92%、3.89%、4.61%、3.67%、3.41%;上述药材均存在一定的抗炎和解热作用,且指标成分含量最高组的药效作用最为明显。结论:采用化学或药效学方法均可对药材质量进行评价,而在药效成分与质控指标成分一致时,两种方法评价结论呈正相关。

Objective： To provide reference for the quality evaluation of Chinese medicine by pharmacological methods. Methods： HPLC was applied to the determination of andrographolide and dehydroanddrographolide in 5batches of Andrographis paniculata （ Burm. f. ） Nees, The model mice of ear swelling was induced by xylene for studying the anti-inflammatory activity of Andrographis paniculata （ Burro. f. ） Nees, and the model of rat fever was induced by lipopolysaccharide （LPS） for studying the antipyretic activity. The correlation between quality control factors and pharmacological elements was studied on the basis of the experimental results. Results： There was difference in the content of andrographolide and dehydroandrographolide. The con- tents were 3.92%, 3. 89%, 4. 61%, 3.67% and 3.41% in the 5batches of Andrographis paniculata （Burro. f. ） Nees respec- tively. M1 the Andrographis paniculata （Burro. f, ） Nees had certain anti-inflammatory and antipyretic activities. The batch of Andrographis paniculata （ Burro. f. ） Nees with highest content of target element had most significant pharmacological activities. Conclusion： Both chemical and pharmacological methods can be used for the evaluation of Andrographis paniculata （Burro. f. ） Nees. In this study, when pharmacological elements and quality control index of Andrographis paniculata （Burro. f. ） Nees are same, the two methods of estimation can be positively correlated.