环孢菌素A-纳米乳促进猪脂肪组织来源干细胞的增殖

Cyclosporin A Nanoparticles Emulsion Promotes Proliferation and Differentiation of Adipose Tissue Derived Mesenchymal Stem Cells

目的评估环孢菌素A-纳米乳(cyclosporine A-nanopaticals emulsion,CsA-NP)对体外脂肪组织来源干细胞(adipose tissue-derived stem cells,ASCs)增殖和分化的影响。方法提取小型猪腹股沟脂肪组织经分离、消化、培养和扩增。ASCs表面标志物经流式细胞仪检测。ASCs增殖和分化采用细胞直接计数和MTT检测。结果检测结果提示ASCs分子表型CD29、CD44、CD90、CD105均呈阳性表达,而CD31、CD34、CD45、HLA-DR均呈阴性表达。CsA-NP浓度在(0.01～1.00mg/ml)之间1～3天对细胞增殖有明显促进作用(P<0.01);其中又以0.5mg/ml的CsA-NP浓度组为最佳。而5mg/ml以上抑制细胞增殖。结论 CsA-NP能促进ASCs增殖和分化,但呈现剂量、时间效应。

Objective To investigated the effect of cyclosporine A - nanopaticals emulsion（CsA - NP） on the proliferation and dif- ferentiation of the adipose tissue - derived stem cells（ASCs） in vitro. Methods ASCs were harvested from porcine inguinal adipose tissue that was isolated and cultured by enzyme digestion and adherent. The molecular phenotypes of ASCs were analysed by flow cytometry. The proliferation and differentiation of ASCs were measnred by direct cell counting and MTTassay, respectively. Results The results showed that ASCs were positive for CD29, CD44, CD90 and CD105, but negative for CD31, CD34, CD45 and HLA - DR. We found that the prolif- eration of ASCs was stimulated by 0. 01 - 1.00mg/ml CsA - NP for 1 - 3days, and the best proliferative effect was observed at concentra- tion of 0.5mg/ml, and significantly decreased by 5mg/ml CsA - NP （ P 〈 0.01 ）. Conclusion Our data demonstrated that CsA - NP may influence both the proliferation and differentiation of ASCs,which dependes on drug concentration and stimulation time.