吡格列酮和高脂喂养对SD大鼠SIRT6表达的影响

Effect of Pioglitazone and High-fat Diet on Expression of SIRT6 in SD Rats

目的观察吡格列酮和高脂喂养对SD大鼠附睾脂肪组织、肝脏和肌肉SIRT6表达的影响。方法 24只雄性SD大鼠随机分为3组:空白对照组(NC)、单纯高脂饮食组(HF)、高脂+吡格列酮组(FP)。RT-PCR及Western blot方法检测附睾脂肪组织、肝脏和肌肉SIRT6表达。结果 (1)与NC组相比,HF组大鼠体重、空腹血糖、胰岛素、甘油三酯、总胆固醇、低密度脂蛋白胆固醇均明显升高(P<0.05),高密度脂蛋白胆固醇则明显下降(P<0.01)。与HF组相比,FP组大鼠体重、空腹血糖、胰岛素、甘油三酯、总胆固醇、低密度脂蛋白胆固醇明显下降(P<0.05)。(2)与正常对照组比较,HF组大鼠附睾脂肪组织、肌肉和肝脏SIRT6 mRNA及蛋白表达轻度下降。吡格列酮治疗后,上述组织SIRT6 mRNA及蛋白表达均显著增高(P<0.05)。结论高脂饮食可诱导大鼠糖脂代谢紊乱,轻度降低SIRT6的表达,吡格列酮改善糖脂代谢紊乱可能通过上调SIRT6的表达。

Objective To observe the effects of pioglitazone and high - fat diet on the expression of SIRT6 in epididymal fat tissue, liver and muscle of SD rats. Methods SD rat models were established with high - fat diet, and 24 male rats were assigned to 3 groups in- eluding high -fat diet（ H F group）, high- fat diet with pioglitazone（ FP group）, and control rats with normal diet（ NC group）. Expressions of SIRT6 in epididymal fat tissue, liver and muscle were detected by RT - PCR and Western blotting. Results （ 1 ） The levels of body weight, fasting blood glucose,insulin,triglycefide,total cholesterol and low -density lipoprotein cholesterol were higher in HF group than in NC group（P 〈 0.05 ）, but high -density lipoprotein cholesterol decreased significangtly. Compared with HF group, body weight, fasting blood glucose,insulin, triglyceride,total cholesterol and low - density lipoprotein cholesterol decreased markedly in FP group （ P 〈 0. 05 ） ; （2） The mRNA and protein expressions of SIRT6 in epididymal fat tissue,liver and muscle were slightly decreased in HE group compared with those in NC group. After pioglitazone treatment, SIRT6 mRNA and protein expression were increased in epididymal fat tissue,liver and muscle（P 〈 0. 05）. Conclusion The SD rats fed with high - fat diets develop glucose and lipid metabolic disorders with slightly de-cresed SIRT6 expressions,Posiglitazone may improv glucose and lipid metabolic disorders by increasing expressions of SIRT6.