牙周治疗前后血清抗体滴度及亲和性的变化

Titer and avidity of serum antibody to Porphyromonas gingivalis before and after periodontal therapy

目的 观察牙周治疗前后抗牙龈卟啉菌 (Porphyromonasgingivalis,Pg)抗体滴度及亲和性的变化 ,探讨其在牙周炎致病机制中的作用。方法 采用ELISA法 ,检测 17例牙周炎患者治疗前及治疗后 1个月及 6名健康对照者的血清抗Pg381IgG抗体滴度及亲和性。结果 牙周炎组治疗前抗体滴度高于对照组 (P <0 0 0 1) ,抗体滴度与探诊深度有负相关趋势 ,治疗后滴度显著降低 (P <0 0 1) ;亲和性与对照组间差异无显著性 ,治疗后也无显著性改变。结论 抗Pg381抗体在牙周炎致病机制中的作用 ,可能主要是“以量取胜”

Objective To observe the changes of titer and avidity of serum antibody to Porphyromonas gingivalis (Pg) before and one month after periodontal therapy, and to observe the correlation of titer and avidity of anti Pg IgG with clinical indices Methods ELISA and DEA ELISA were used to determine the titer and avidity of serum IgG antibody to Pg 381 before and one month after periodontal treatment in 17 periodontitis patients and 6 healthy control subjects Results The titers of anti Pg IgG antibody were higher in periodontitis patients than in healthy control subjects ( P <0 001) All patients were seropositive (titers to Pg were higher than 2 fold above the median for control subjects) IgG antibody avidity to Pg in periodontitis patients were not found to be significantly lower than that in control group The titers to Pg decreased significantly at one month after periodontal therapy, but antibody avidity didn't change Serum anti Pg antibody titers had a tendency of negative correlation with probing depth The antibody titers were significant higher in periodontitis patients with ≤5 mm mean probing depth than in patients with >5 mm mean probing depth Conclusions It is suggested that the humoral immune response to Pg 381 is protective and this protective effect mainly depends on the titer of antibody