巨细胞病毒与复发性阿弗他溃疡T细胞亚群关系的研究

Cytomegalovirus infection and the changes of T-lymphocyte subsets in RAU patients

目的 了解不同时期复发性阿弗他溃疡 (recurrentaphthousulcer,RAU)患者外周血中T细胞亚群数量的变化 ,探讨巨细胞病毒对RAU患者细胞免疫的影响。方法 采用APAAP法 ,分别对2 6例溃疡期和 10例间歇期RAU患者外周血CD4 (辅助性 /诱导性 )和CD8(抑制性 /杀伤性 )细胞进行检测。结果 溃疡期CD4 、CD8细胞百分率分别为 36 19%和 2 9 38% ,CD4 /CD8细胞比为 1 2 8,其中 7例CD4 /CD8细胞比例倒置 ,占 2 6 92 % ;间歇期CD4 、CD8细胞百分率分别为 39 90 %和 34 2 1% ,CD4 /CD8细胞比为 1 17。溃疡期CD4 、CD8细胞及间歇期CD4 细胞均低于正常值 (P <0 0 5 ) ,间歇期CD8细胞与正常值差异无显著性 (P >0 0 5 ) ,间歇期CD3 (总T细胞 )、CD4 、CD8细胞均有不同程度增高 ,11例人巨细胞病毒 (humancytomegalovirus,HCMV)DNA阳性患者与 9例HCMVDNA阴性患者比较 ,阳性组CD4 细胞低于阴性组 ,差异有显著性 (P <0 0 5 )。结论 持续性细胞免疫功能失调是RAU发病过程的一个重要环节 。

Objective To study the quantitative changes of the T lymphocyte subsets in RAU patients with different stages and to know the influences of cytomegalovirus(CMV) on cellular immunity in RAU patients Methods CD 4(helper/inducer) and CD 8 (suppressor/cytotoxic) cells in the peripheral blood were tested with monoclonally immunocytochemical technique (APAAP method) in 26 ulcerative stage patients and 10 inactive patients Results The percentages of CD 4 and CD 8 cell were 36 19% and 29 38% respectively in ulcerative stage The ratio of CD 4/ CD 8was 1 28 and it was inverted (<1 0) in 26 92% of the patients The percentages of CD 4 、CD 8 cells was 39 90% and 34 21% in inactive stage respectively The ratio of CD 4/ CD 8 was 1 17 The number of CD 4 and CD 8 cells in ulcerative stage and CD 4 cells in inactive stage was significantly lower than the normal values, P <0 05 The number of CD 8 cells in inactive stage was not significantly different compared with the normal value However the numbers of CD 3(total T cells)、CD 4 and CD 8 cells increased to some extent in inactive stage CD 4 cells was significantly lower in 11 human cytomegalovirus (HCMV) DNA positive patients than that in 9 HCMV DNA negative patients ( P <0 05 ) Conclusions A permanent disorder of cellular immunity was an important part of pathogenesis in RAU CMV infection may be an important factor resulting in the disorder of cellular immunity because of the imbalance of T lymphocyte subsets