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甲基汞对大鼠脑皮质谷氨酰胺合成酶的影响

Effect of methylmercury on glutamine synthetase in rat brain cortex
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摘要 取Wistar大鼠36只,按体重随机分成3组,每组12只。第1～3组分别为对照组、低甲基汞(MeHg)组和高MeHg组。每日染毒1次,连续4周。每周测量大鼠体重,用冷原子吸收法测量大鼠脑皮质内Hg含量,比色法测定GS活力、谷氨酸(Glu)和谷氨酰胺(Gln)含量,用Real-time PCR和Western blotting检测GS mRNA表达和蛋白水平。与对照组比较,在低MeHg组中Hg含量明显升高,GS活力、GS蛋白水平均显著下降;在高MeHg组中,大鼠体重、GS活力、Gln含量、GSmRNA表达和蛋白水平均明显下降,Hg和Glu含量均显著增高。提示甲基汞能导致大鼠脑谷氨酰胺合成酶表达和功能异常,谷氨酰胺合成酶在甲基汞诱导的神经毒性过程中起重要作用。
作者 邓宇 徐兆发 魏衍刚 刘巍 杨海波 徐斌
机构地区 中国医科大学公共卫生学院环境卫生学教研室
出处 《中国工业医学杂志》 CAS 北大核心 2012年第6期434-436,共3页 Chinese Journal of Industrial Medicine
基金 国家自然科学基金资助项目(81172631)
关键词 甲基汞 谷氨酰胺合成酶 神经毒性
分类号 R994.6 [医药卫生—毒理学]
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参考文献11

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二级参考文献9

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中国工业医学杂志
2012年 第6期

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