聪智灵对拟血管性痴呆小鼠海马乙酰胆碱酯酶的影响

Effect of Congzhiling on Hippocampus AchE of Mice with Pseudo-VD

目的:观察聪智灵对血管性痴呆(VD)模型小鼠脑组织海马区乙酰胆碱酯酶(AchE)活性及学习记忆能力的影响,探讨聪智灵对血管性痴呆的治疗效应及作用机制。方法:采用反复结扎双侧颈总动脉的方法复制VD模型,随机分为假手术组、模型组、聪智灵高剂量组、聪智灵低剂量组、尼莫地平组。造模当天动物苏醒后开始治疗,灌胃给药15 d后,采用跳台法检测小鼠学习记忆能力变化,分光光度法检测海马组织AchE活力。结果:模型组小鼠学习与记忆成绩较假手术组均显著降低(P<0.01),各用药组小鼠学习与记忆成绩较模型组均显著提高(P<0.05或P<0.01);模型组小鼠海马组织AchE活性明显低于假手术组(P<0.01),各用药组小鼠海马组织AchE活性显著高于模型组(P<0.05或P<0.01)。结论:聪智灵具有保护脑缺血再灌注损伤的作用,其机制可能与提高海马组织内AchE活性、减轻脑缺血再灌注对海马区神经元损伤有关。

Objective: to observe the effect of Congzhiling on the AchE activity and learning and memory ability of mice with VD ( vascular dementia) ,and to explore the therapeutic effect and the functional mechanism of Congzhiling. Methods: VD models were made by repeatedly ligating the bilateral carotid artery,which were randomly divided into sham operation group,model group,high dose of Congzhiling group,low dose group and Nimodipine group. Treatment began on the day of model-making when the animals woke. After 15 days of intragastric administration,jumping stand was taken to detect the changes of learning and memory ability of the mice,and spectrophotography was taken to detect AchE activity. Results: As for the learning and memory ability,model group decreased significantly than sham operation group( P < 0. 01) ,while treatment groups increased significantly than model group ( P < 0. 05 or P < 0. 01) ; As for hippocampus AchE activity,model group was significantly lower than sham operation group ( P < 0. 01) ,while treatment groups were significantly higher than model group( P < 0. 05 or P < 0. 01) . Conclusion: Congzhiling is able to protect the ischemia-reperfusion injury,whose mechanism may be related to improving hippocampus AchE activity,lessening the ischemia-reperfusion injury on the hippocampus neurons.