应用亚硫酸钠快速制备低氧性肺血管收缩离体模型所需低氧性Krebs-Henseleit液的方法及评价

Rapid preparation of hypoxic Krebs-Henseleit solution with sodium sulfite for in vitro model of hypoxic pulmonary vasoconstriction

目的:探讨应用亚硫酸钠(sodium sulfite,Na2SO3)快速去氧制备低氧性肺血管收缩(hypoxia pul-monary vasoconstriction,HPV)离体模型所需低氧性Krebs-Henseleit(KH)溶液的可行性。方法:在暴露于空气的开放环境中将不同剂量Na2SO3加至0.5 L温度为37℃的KH溶液中,用i-STAT便携式临床分析仪检测加样1min后溶液pH值、氧分压(PO2)、二氧化碳分压(PCO2)和钠离子(Na+)浓度的变化;选择适合HPV模型条件的Na2SO3剂量溶入0.5 L KH液后,检测此低氧溶液在1、10、20、30、60和90 min时上述指标的变化。结果:大于0.2g(包括0.2 g)Na2SO3加至0.5 L KH液中可降低KH液的PO2(P<0.01),且随着剂量的增加,PO2降低。其中,1.5 g Na2SO3剂量可使0.5 L KH液达到HPV模型所需的低氧状态PO2(20～40 mmHg)并维持此低氧状态至少90min,溶液pH值、PCO2和Na+浓度均处于正常范围。结论:应用Na2SO3可制备HPV离体模型所需的低氧溶液,方法简单,快速可行,状态稳定,无需密闭。

AIM： To investigate the feasibility of using sodium sulfite （Na2SO3 ） to prepare hypoxic Krebs - Henseleit （KH） solution for hypoxic pulmonary vasoconstriction （HPV） model in vitro. METHODS： Different doses of Na2SO3 were added into 0.5 L KH solution at 37 ℃. An i - STAT portable clinical analyzer was used to measure the oxy- gen partial pressure （ PO2 ）, carbon dioxide partial pressure （ PCO2 ）, pH value and the concentration of sodium （ Na＋ ） in these KH - Na2SO3 solutions 1 min after administration. Then the dose of Na2SO3 suitable for HPV model was dissolved in 0.5 L KH solution and the above indexes in the solution were monitored at various time points at 37 ℃ under atmospheric pressure. RESULTS ： More than 0.2 g （ including 0.2 g） Na2SO3 reduced the PO2 of 0.5 L KH solution in a dose - de- pendent manner （P 〈0.01 ）. In addition, 1.5 g Na2SO3 reduced the PO2 of 0.5 L KH solution to 20 - 40 mmHg and maintained the hypoxic state for at least 90 min （suitable for HPV model in vitro）, but had nearly no effect on the PCO2, pH and Na＋ levels. CONCLUSION： The hypoxia solution for HPV model could be reached by Na2SO3 in open air and the method is simple, easily feasible and stable.