应用原位杂交和细胞遗传学方法检测淋巴瘤患者的染色体畸变

Detection of chromosome aberration of patients with lymphoma by in situ hybridization and cytogenetic technology

目的：检测淋巴瘤患者的染色体畸变。方法：运用细胞遗传学方法检测１５例淋巴瘤初发病人的外用血淋巴细胞。运用原位杂交技术检测２１例淋巴瘤患者的病变淋巴结７号三体（＋７）和 １２号三体（＋１２）的发生率，其中１２例与外周血染色体标本为同一患者。结果：外周血染色体分析表明：自然状态下和丝裂霉素Ｃ诱导下，淋巴瘤组的染色体断裂率和细胞畸变率均比正常对照组高。原位杂交检测发现２１例中５例有＋７（２３. ８％），３例有＋１２（ １４．３％）。结论：淋巴瘤患者外周血淋巴细胞染色体具有不稳定和易断裂的特点。＋７和＋１２是淋巴瘤常见的染色体畸变。

Objective: To detect chromosome aberration of patients with lymphoma. Methods: Peripheral lymphocytes of 15 patients who were pathologically diagnosed as primary lymphoma were detected by cytogenetic technology. Lymphoid nodes of 21 lymphomas cases were detected by in situ hybridization to get the incident rate of +7 and +12. Among 21 cases, 12 cases were the same patients of peripheral samples. Results: Both in nature and MMC (mitomycin C ) condition, chromosome breakages rate and percentages of aberrant cell in patients of lymphomas were higher than that in control group. Using in situ hybridization, +7 was detected in 5 cases of 21(23.8% ), +12 was detected in 3 cases (14.3% ). Conclusions: There are chromosome instability and easiness of break in peripheral blood lymphoeytes of lymphoma patients. Trisomy 7 and trisomy 12 are common chromosome aberrations in lymphoma.