乳腺癌雌激素受体表达与p53、PCNA、AgNOR表达关系

The relation of estrogen receptor expression with p53, PCNA expression and AgNOR count in breast carcinoma

目的：研究乳腺癌雌激素受体（ＥＲ）表达与ｐ５３，增殖细胞核抗原（ｐｒｏｌｉｆｅｒａｔｉｏｎ ｃｅｌｌ ｎｕｃｌｅａｒ ａｎｔｉｇｅｎ，ＰＣＮＡ）表达和银染核仁组成区（ＡｇＮＯＲ）计数关系。方法：采用ＡｇＮＯＲ染色技术和免疫组化Ｓ－Ｐ法，用抗ＥＲ，抗ｐ５３（ＤＯ－７）及抗ＰＣＮＡ（ＰＣ１０）单克隆抗体，研究５０例乳腺癌ＡｇＮＯＲ计数及ＨＲ，ｐ５３．ＰＣＮＡ表达。结果：乳腺癌３３例（６６％）ＥＲ表达阳性，１８例（３６％）；ｐ５３蛋白表达阳性，乳腺癌ｐ５３表达与ＥＲ阴性有相关关系（Ｐ＜０.０１）；ＥＲ阳性组较ＥＲ阴性织组ＰＣＮＡ表达及ＡｇＮＯＲ值均较低，且相差有显著性（Ｐ＜０.０５）。结论：ｐ５３表达可能抑制乳腺癌ＥＲ分化；ＥＲ阳性较ＥＲ阴性病例癌细胞增殖活性低。

Objective: To investigate the relation of estrogen receptor(ER) with the expression of p53, proliferation cell nuclear antigen(PCNA), and AgNOR count in breast carcinoma. Methods: AgNOR count,ER,p53 and PCNA expression were detected by using silver-staining method and S-P immunohistochemical staining with antibodies for ER,p53 (DO-7 ) and PCNA (PC10) in 50 cases of paraffin-embedded breast carcinoma.Results: A total of 18 cases (36% ) showed p53 protein expression and 33 cases (66% ) showed ER expression, expression,there was significant association of ER negative expression with p53 positiveexpression.In the ER negative group,the PCNA high expression and the average values of AgNOR were significant higher than those in the ER positive cases.Conclusion:p53 expression might inhibit the ER expression in breast carcinoma.There is a higher cellular proliferation activity in ER negative cases than in ER positive cases.