板栗果实糖和淀粉积累及相关酶活性关系的研究

Changes in Sugar,Starch,Some Enzymes Involved and Their Relationships During the Development of Chinese Chestnut

以6年生板栗‘3113’为试材,采用比色法对果实发育过程中可溶性总糖、蔗糖和淀粉含量及相关酶活性的相互关系进行了研究。结果表明:果实横、纵径生长规律呈双"S"型,单苞质量呈"S"型曲线。可溶性总糖含量呈上升趋势,蔗糖含量呈下降趋势;淀粉含量呈单峰曲线。蔗糖合成酶(SS)和蔗糖磷酸合成酶(SPS)活性在花后60d达到峰值,之后趋于下降,与蔗糖含量变化趋势吻合;ADPG焦磷酸化酶(AGPPase)、水溶性淀粉合成酶(SSS)、颗粒结合型淀粉合成酶(GBSS)的活性均呈单峰曲线,但最高峰的时间略有差异。相关分析表明,可溶性淀粉与可溶性总糖呈极显著的正相关,相关系数为0.978;SS和SPS活性与蔗糖存在极显著正相关,相关系数分别为0.905和0.892;可溶性淀粉含量与SSS活性呈显著正相关,与GBSS活性呈极显著正相关,相关系数分别为0.764和0.883。

This research applied colorimetric method to explore the interactions of the total soluble sugar content, sucrose content, starch content and the relevant enzyme activity of Chinese ＇ 3113 ＇ chestnut in the fruit establishment stage. The starch accumulation sucrose metabolism and relevant enzymes activities were investigated in fruit establishment stage of Chinese chestnut. The results indicated that the transverse and longitudinal diameters show double ＂S＂ type, the average weight of Chinese chestnut showed ＂S＂ curve. The total soluble sugar content showed an increasing tend0ncy, sucrose content had decreasing tendency, starch content presented as single, peak curve, SS and SPS activities which reached the peak point 60 days after flowering, decreased afterward, showed the identical tendency as sucrose variation. All the activity variation of the AGPP, SSS and GBSS presented as single peak curve, but the time were not the same. According to the correlation analysis, soluble starch content which positive correlated to total sugar content significantly, the correlation coefficient was 0.978. The activity of SS and SPS positive correlated to sucrose content significantly, the correlation coefficients were 0.905 and 0.892, respectively. The content of soluble starch positive correlated to SSS and significantly positive correlated to GBSS, the corresponding coefficients were 0.764 and 0.883, separately.