中国野生白灵菇遗传多样性的SCoT分析

SCoT Analysis of Genetic Polymorphism for Wild Germplasm of Pleurotus eryngii var. tuoliensis from China

对来自新疆裕民、托里、青河等3个县的63株野生白灵菇(Pleurotus eryngiivar.tuoliensis)的遗传多样性进行了SCoT分析。9条SCoT引物共产生147条带,多态性条带比率为92.52%,平均多态性条带数为15.11。总样本的Nei’s基因多样性指数为0.261,Nei’s遗传一致度0.612～0.932,平均为0.772,这表明中国野生白灵菇具有丰富的遗传多样性。聚类分析和主坐标分析结果高度相似,几乎将供试样本完全按地理分布划分为3大类群,表明野生白灵菇的遗传多样性与地理分布密切相关。遗传距离分析表明,3个居群中,裕民居群与托里居群的亲缘关系最近,遗传距离为0.086;托里居群与青河居群的亲缘关系最远,遗传距离为0.156。相关性分析表明,遗传距离与直线地理距离和经向地理距离均不存在相关性,而与纬向地理距离存在明显的正相关关系。

The genetic diversity was analyzed by start codon targeted （SCOT） markers for 63 wild strains ofPleurotus eryngii var. tuoliensis collected from three counties in Xinjiang Autonomous Region, China. A total of 147 bands were generated from nine SCoT primers, of which 92.52% was polymorphic, and each primer generated 15.11 bands in average. Nei＇s genetic diversity index was 0.261. The similarity coefficient ranged from 0.612 to 0.932, and the average 0.772. These results revealed rich genetic diversity in the wild resources of P. eryngii vat. tuoliensis. Principal coordinate analysis was identical to the cluster analysis. All the samples could be divided into three groups with their geographical origins, which indicated the genetic diversity related closely to geographical distribution for this organism. With the genetic distances assay, the genetic relationship for the samples collected from Yumin and Tuoli was the closest （GD = 0.086） and that from Tuoli and Qinghe was the farthest （GD = 0.156） among the three populations. Correlation analysis revealed latitude distance, rather than longitude or geographical distance, was related to genetic distance.