牡丹89个不同种源品种遗传多样性和亲缘关系分析

Assessment of Genetic Diversity and Relationship of 89 Tree Peony Cultivars from Different Provenances

利用ISSR分子标记技术,从121条ISSR备选引物中筛选出条带清晰、多态性好的19条引物对89个牡丹品种的遗传多样性进行了分析。通过优化PCR试验条件,共获得188条带,其中177条表现出多态性,多态位点的百分率为94.1%。通过计算机软件分析,供试牡丹品种平均有效等位基因数为1.514,平均Nei’s基因多样性指数为0.3085,平均Shannon’s信息指数为0.4713;各品种间的相似系数介于0.3294～0.7744之间,平均为0.5722。利用UPGMA法作图,供试的89个品种可聚为2个类群,很好地将不同来源的品种区分开来。

In this paper, ISSR-PCR was used to detect the genetic diversity of these peony cultivars. Nineteen good ISSR primers selected from 122 primers were used to amplify the genomic DNA of some introduced peony cultivars to study on their genetic diversity. The results showed that 188 bands were detected, of which 177 were polymorphic with the polymorphism frequency of 94.1%. Analyzing these results by POPGENE and NTSYS, it was found that the average value of effective allele number, Nei＇s gene diversity and Sharmon＇s information index of the detected peony cultivars were 1.514, 0.3085 and 0.4713, respectively, and the similarity coefficient were ranged from 0.3294 to 0.7744. The 89 tree peony cultivars could be separated into 2 groups by UPGMA （unweighted pair group method with arithmetic average） analysis. The results also provide references for the peony genetic breeding.