祁连山东段高寒草甸植物总酚和黄酮含量及抗氧化活性研究

Contents of Total Polyphenols and Flavonoids from Alpine Plants from Qilianshan Mountains and Their Antioxidant Activity

采用Folin-Ciocalteu法和铝盐显色法对祁连山东段12种高寒植物(灌木嫩枝和草本植物叶片)进行总酚、黄酮含量的测定,并用FRAP法和ABTS法测定其总抗氧化能力。结果表明:(1)金露梅、山生柳、高山绣线菊、西藏沙棘、珠芽蓼的总酚和总黄酮含量较高且总抗氧化能力较强,其中山生柳的总酚含量最高为19.58%,高山绣线菊的黄酮含量最高为13.23%。(2)采用FRAP法测得西藏沙棘的抗氧化能力最强为49.58mmol/L,而用ABTS法测得金露梅的抗氧化能力最强为32.46mmol/L。(3)总酚、总黄酮与总抗氧化能力(FRAP法)具有极显著的相关性(相关系数分别为0.887 1和0.915 7)。分析认为,12种高寒植物的植株体内总酚和总黄酮含量越高,其抗氧化能力越强。

The contents of total polyphenols and flavonoids and their influence on the antioxidant acttvlty of 12 alpine plants sampled from Qilianshan Mountains were investigated. The determining was focused on the shoots and leaf of the targeted plants by using methods of The Folin-Ciocalteu method and aluminum salt colour reaction. The total antioxidant capacity was estimated by ABTS and FRAP methods. The results showed that Potentilla fruticosa , Salix oritrepha , Spiraea alpina , Hippophae thibetana and Polygonum viviparurn had high levels of total polyphenols, flavonoids and antioxidant capacity. The highest total poly- phenols content was found in the S. oritrepha,it was as high as 19.58%. The total fiavonoids content of S. atpina was the highest,up to 13.23%. While the H. thibetana showed a strongest antioxidant capacity of 49.58 mmol/L（FeSO4 ） by FRAP measurement,but P. fruticosa had the strongest antioxidant capacity for 32.46 mmol/L（Trolox） by ABTS determination. Total polyphenols,flavonoids and total antioxidant capac- ity in the FRAP method had extremely significant correlation; the relative coefficient reached a value of 0. 887 1 and 0. 915 7 ,respectively. The analysis about 12 alpine plants showed that the higher total polyphenolsand flavonoids,the stronger total antioxidant capacity.