松醇油降解菌的分离鉴定及降解条件优化

Isolation and identification of pine oil degrading bacteria and optimization of degrading conditions

从广东凡口铅锌矿尾矿库周边土壤中分离出一株能高效降解松醇油的细菌KS-1,并进行人工配制含松醇油的模拟废水的化学需氧量(COD)降低条件优化试验研究。形态学和生理学分析表明:该KS-1菌株为革兰氏阳性好氧菌,呈细胞杆状、芽孢椭圆或柱状;接触酶、硝酸盐还原和V-P测定呈阳性反应;能利用葡萄糖、木糖、甘露醇、柠檬酸盐、淀粉和明胶生长,不能利用吲哚、卵黄卵磷脂酶、苯丙氨酸脱氨酶和丙酸盐生长。16SrDNA序列分析表明:该菌株与Bacillus subtilisAF0907的相似性达到100%,最终鉴定菌株KS-1为枯草芽孢杆菌(Bacillussubtilis AF0907),登录号为GU272021。正交试验优化结果表明:菌株KS-1降低松醇油废水COD工艺最佳条件为pH4,接种量15%,摇床转速200 r/min,松醇油浓度300 mg/L,在此条件下反应4 d,COD去除率达到65.91%。

A strain KS-1 capable of highly degrading pine oil was isolated from the soil collected from tailings reservoir in Fankou Lead-zinc Mine of Guangdong province,and the best degradation conditions of pine oil wastewater（chemical oxygen demand,COD） were investigated.The analysis of morphology and physiology shows that the strain KS-1 is gram-positive and aerobic bacterium and rhabditiform,which can grow by using glucose,xylose,mannitol,citrate,starch and gelatin and cannot grow with indole,yolk lecithinase,phenylalanine deaminase and propionate.Based on analysis of phenotype,physiological and biochemical characters and 16SrDNA,KS-1 is identified as Bacillus subtilis AF0907 due to its 16SrDNA sequence,GU272021,which has the similarity of 100% to Bacillus subtilis AF0907.According to the orthogonal optimization test,the degradation conditions are as follows： pH 4,inoculating ratio 15%,rotation speed 200 r/min,pine oil concentration 300 mg/L,with COD removal rate up to 65.91% in 4 d.