摘要
目的:探讨维甲酸灌注法对不同月龄雌性SD大鼠骨质疏松动物模型的诱导效果。方法:挑选不同月龄SD雌性大鼠,使用维甲酸80mg/(kg·d)对实验动物灌胃给药20天,在实验开始第30天、第45天、第60天对大鼠进行全身骨密度测定。结果:3月龄和6月龄大鼠在实验第30天和第45天骨密度呈逐渐恢复趋势,但是与对照组比较有显著性差异,在实验第60天骨密度与对照组比较均无显著性差异。9月龄大鼠在实验后骨密度恢复较为缓慢,在第60天骨密度仍然与对照组有显著性差异。结论:使用维甲酸诱导骨质疏松大鼠模型,从远期效果以及骨量丢失维持时间方面考虑,9月龄成熟大鼠优于3月龄、6月龄大鼠,适合用于骨质疏松造模进行后续动物实验。
Objective To observe the induction effect of retinoic acid perfusion on osteoporotfc (OP) models in female SD rats of different ages. Methods Female SD rats of different ages were involved in the study. Rats were treated with retinoic acid 80 mg (kg*d) by gastrogavage dosing for 20 days. Bone mineral density (BMD) was detected at the 30,45 and 60 days after dosing. Results At the 30 and 45 days, BMD was gradually inclined to normal in groups of 3-month and 6-month rats, which showed significant difference compared with the control group. There was no significant difference between the group of 60 days and control group. BMD of 9-month rat showed slow recovery after dosing, which showed significant difference compared with the control group. Conclusion Considering long-term effect and continuing bone loss, OP models of 9-month rat was superior to that of the 3-month and the 6-month rat induced by retinoic acid, which was appropriate to construct OP models for the follow-up animal experiment.
出处
《中国美容医学》
CAS
2012年第12期2209-2210,共2页
Chinese Journal of Aesthetic Medicine
基金
兰州军区医药卫生计划项目资助(项目编号:CLZ12JB28)
关键词
骨质疏松症
维甲酸
骨密度
大鼠
osteoporotic(OP)
retinoic acid
Bone mineral density(BMD)
rat