RT-PCR一步法检测慢性粒细胞白血病bcr/abl融合基因

Detection of bcr/abl fusion gene in chronic myelogenous leukemia by single step reverse transcription polymerase chain reaction

应用RT PCR一步法检测了 42例慢性粒细胞白血病 (CML)患者的bcr/abl融合基因 ,均显示bcr/abl基因阳性 ,其中2 1例为 16 8bp扩增带 ,18例为 93bp扩增带 ,3例同时具有 16 8bp和 93bp二条扩增带。bcr/abl融合基因检测 ,对CML的诊断、治疗及预后判断均有重要意义。RT PCR一步法特异性好 ,敏感性高 ,简单快速 ,可节省试剂 ,值得推广应用。

To detect bcr/abl fusion gene of 42 patients with chronic myelogenous leukemia(CML)by single step reverse transcription polymerase chain reaction(RT PCR).It showed all were bcr/abl positive.21 of 42 cases showing an amplifying band of 168 bp,18 showing 93 bp,3 showing both 168 bp and 93 bp.The detection of bcr/abl fusion gene is significant in diagnosis,treatment and prognoses of CML.The method of one step RT PCR is specific,sensitive,simple,rapid,economic and worthy of spreading.