摘要
目的:评价Bio-Gide生物膜与人牙周膜干细胞(periodontal ligament stem cells,PDLSCs)的生物相容性,为其作为细胞载体用于牙周组织工程提供依据。方法:体外分离培养人PDLSCs,经鉴定后与Bio-Gide生物膜浸提液共同培养,评价该膜材料的细胞毒性;将人PDLSCs接种于Bio-Gide生物膜后,通过扫描电镜和Hochest荧光核染色观察其在膜材料上的粘附、生长情况。结果:人PDLSCs和Bio-Gide生物膜浸提液共同培养后,其增殖率为122.66%,无细胞毒性;人PDLSCs接种于膜材料上培养1～12 d后扫描电镜观察可见,PDLSCs在膜材料上能正常增殖且成膜性生长;经Hochest荧光核染色观察人PDLSCs与膜材料的粘附率为86.7%。结论:Bio-Gide生物膜除具有良好的物理屏障外,无细胞毒性,且能支持PDLSCs的成膜性生长。
AIM： To explore the possibility of using Bio-Gide membrane as cell vehicle for periodontal tis- sue engineering, we evaluated the biological properties of Bio-Gide membrane using human periodontal stem cells （PDLSCs）. METHODS： Human PDLSCs were cultured and characterized, and co-cultured with the lixiviated solu- tion of Bio-Gide membrane. Cytotoxicity of the lixiviated solution of Bio-Gide membrane was evaluated by tetrazolium salt [ MTY, 3 -（4,5 -dimethylthiazol- 2- yl） -2,5-diphenyhetrazolium bromide ] assay. Cell attachment and pro- liferation of human PDLSCs were observed using SEM and fluorescence nuclear staining. RESULTS：No cytotoxicity was found with the lixiviated solution of Bio-Gide membrane, and the rate of cell proliferation of PDLSCs on membrane was 122.66%. The muhiplicate growth of PDLSCs on the Bio-Gide membrane was detemined by SEM observations, and a multiple cell sheet on Bio-Gide membrane was formed. The adherence rate of the PDLSCs on the Bio-Gide mem- brane was 86.7%. CONCLUSION： Bio-Gide membrane shows good cell biological performance and cell compatibili- ty. It has no cytotoxicity on seeding PDLSCs, and supports PDLSCs to form cell membrance, suggesting a potential ap- plication of Bio-Gide membrance for cell delivery.
出处
《牙体牙髓牙周病学杂志》
CAS
北大核心
2012年第12期681-685,724,共6页
Chinese Journal of Conservative Dentistry
基金
国家自然科学基金资助项目(81071253/H1818
31170912/C1002)
