产油微藻筛选和鉴定及其产油性能的研究

Study on Screening and Identification of Oleaginous Microalgae and Its Oil-producing Charateristic

为了筛选具有产油能力的微藻,从自然界水体中分离出14株微藻,根据形态特点对它们进行了初步鉴定。对其中12株微藻在自养和异养条件下的生长特性和产油性能进行了比较。通过微藻的生长曲线,生物量和油脂含量等指标,从中筛选出高产藻株并对该藻株进行了分子生物学鉴定。结果表明:藻株Y06在12种微藻中的油脂产量和产率最高,经18S rDNA鉴定确定为栅藻(Scenedesmus abundans)。藻株Y06在自养条件下的油脂产率为9.40 mg/(L.d),在异养条件下的油脂产率为201.29 mg/(L.d)。

In order to screen microalgae that has the potential of oil production, fourteen strains of microalgae were isolated from the natural water and preliminarily identified by their morphological characteristics. Growth characteristics and lipid production capacity of twelve strains of microalgae were compared under autotrophic and heterotrophic conditions. High-yield strains were identified by molecular bioteehnology after being screened by the growth curve of microalgae, biomass and fat content and other indicators. The following results were obtained： Y06, one of the 12 species of microalgae, which was identified as Scenedesmus abundans by 18S rDNA analysis, has the highest oil production and productivity. The lipid productivity of Y06 were 9.40mg/L. d and 201.29 mg/L. d under autotrophic and heterotrophic conditions, respectively.