枇杷叶荚蒾的愈伤组织诱导及植株再生

Callus Induction and Plantlet Regeneration of Viburnum rhytidophyllum Hemsl.

以枇杷叶荚蒾幼叶为外植体,MS为基本培养基,研究不同种类和浓度的植物生长调节物质对愈伤组织诱导、分化及生根的影响,建立了枇杷叶荚蒾再生体系。结果表明:枇杷叶荚蒾最佳灭菌组合为75%酒精预处理20s,再用0.1%HgC12浸泡12min;最佳愈伤组织诱导培养基为MS+6-BA1.0mg·L-1+NAA0.25mg·L-1+2,4-D1.0mg·L-1,诱导率最高达92%;最适芽分化培养基为MS+6-BA1.5mg·L-1+NAA0.2mg·L-1,分化率达87.25%;适宜的生根培养基为1/2MS+NAA1.0mg·L-1,生根率达85%。

The young leaves of Viburnum rhytidophyllum were taken as explants, and MS was basic medium. The effects of different concentrations and combinations of plant growth regulators on callus induction, bud differentiation and root formation were studied. Tissue culture regeneration system for V. rhytidophyllum was established. The results showed that the best sterilization method for materials was to soak explants into 0.1% HgC12 solution for 12 min after pretreatment with 75% alcohol for 20 s; the best medium for callus formation was MS＋6-BA 1.0 mg·L-1＋NAA 0.25 mg·L%2,4-D 1.0 mg·L-1, callus induction rate reached 92%; the optimum bud differentiation medium was MS＋6-BA 1.5 mg·L-1＋NAA 0.2 mg·L-1 with the induction rate of 87.25%; the suitable medium for root induction was 1/2MS＋NAA 1.0 mg·L-1 and the rooting rate was 85%.