应用基因表达谱芯片研究高果糖大鼠胰岛素抵抗相关基因

Study on Insulin Resistance-Associated Gene in Fructose-Fed Rats by cDNA Microarray

目的比较正常大鼠和高果糖大鼠骨骼肌组织基因表达差异,探讨胰岛素抵抗的发病分子机制。方法实验大鼠分为正常对照组和高果糖组;从大鼠骨骼肌组织抽提mRNA,经逆转录分别用Cy3、Cy5荧光标记,获得两组动物来源cDNA探针;cDNA探针与4096条大鼠cDNA基因表达谱芯片杂交,扫描,重复2次实验,通过计算机数据处理。结果筛选出胰岛素抵抗差异表达的基因共140条,基因上调有62条,基因下调有78条。结论从骨骼肌组织中筛选出大量的差异表达基因,这些基因可能参加了胰岛素的发生发展过程。

Ahn To study the expression of insulin resistance-associated gene and to elucidate the molecular mechanisms in skeletal muscle tissue between fructose-fed rats and normal rats with cDNA microarray. Methods The rats were divided into two groups, one was normal and the other was fructose-rich chow. The mRNA was isolated and pu- rified from skeletal muscle tissue of two groups. The mRNA from two kinds of tissue was reverse transcribed to cDNA with Cy3-dUTP and CyS-dUTP separately to prepare hybridization probes. The mixed probes were hybridized to cDNA microar- ray. The microarray was scanned , analyzed and repeated for two times. Results Among the total 4096 tested genes, 140 genes were differently expressed,62 up-regulated,78 down-regulated. Conclusions Gene expression pro- file is different from skeletal muscle tissue between fructose-fed rats and normal rats. The differential expression of genes may be related to the pathogenesis of insulin resistance.