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胃癌有丝分裂关卡基因BUB1和BUBR1 mRNA的表达

Expressions of mitotic checkpoint gene BUB1 and BUBR1 mRNA in gastric cancer
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摘要 目的研究胃癌组织中有丝分裂关卡基因BUB1和BUBR1的表达。方法采用半定量RT-PCR技术检测39例胃癌组织及正常胃组织中BUB1及BUBR1基因的表达。结果胃癌组织中BUB1mRNA/β-actin mRNA和BUBR1mRNA/β-actin mRNA拷贝数比值均明显高于正常胃组织(1.84±0.43 vs. 0.86±0.44和2.00±1.36 vs. 0.96±0.60)(P<0.05)。结论 BUB1基因和BUBR1基因在胃癌中高表达可能与癌细胞的异常增殖有关。 Objective To study the expressions of mitotic checkpoint gene BUB1 and BUBR1 mRNA in gastric cancer. Methods Using semi-quantitative PCR, the expressions of mitotic checkpoint gene BUB1 and BUBR1 mRNA were detected in 39 gastric cancer samples（group A） and the tumor-adjacent normal tissues（group B）. Results The ratios of BUB1/β-actin gene rnRNA copies and BUBR1/β-actin gene mRNA copies in group A were significantly higher than those in group B （1.84±0.43 vs. 0.86±0.44 and 2.00±1.36 vs. 0.96±0.60）（P〈0.05）.Conclusion BUB1 and BUBR1 genes are highly expressed in gastric cancer, which may be associated with abnormal proliferation of cancer cells.
作者 余晓峰 冯全林 朱震 陈海军 王雪林 徐正平 钱宏亮 吴江 吴清 陆琦
机构地区 昆山市中医医院肿瘤外科
出处 《江苏医药》 CAS CSCD 北大核心 2012年第24期2978-2980,共3页 Jiangsu Medical Journal
关键词 胃癌 有丝分裂关卡基因 BUB1 BUBR1 Gastric cancer Mitotic checkpoint genes BUB1 BUBR1
分类号 R735 [医药卫生—肿瘤]
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参考文献11

  • 1Huang H,Hittle J,Zappacosta F,et al. Phosphorylation sites in BubR1 that regulate kinetochore attachment, tension, and mitotic exit[J]. J Cell Biol,2008,183 (4) :667-680.
  • 2Sczaniecka M, Feoktistova A, May KM, et al. The spindle checkpoint functions of Mad3 and Mad2 depend on a Mad3 KEN box-mediated interaction with Cdc20-anaphase- promoting complex (APC/C) [J]. J Biol Chem, 2008, 283 (34) :23039-23047.
  • 3Davenport J, Harris LD, Goorha R. Spindle checkpoint function requires Mad2-dependent Cdc20 binding to the Mad3 homology domain of BubR1 [J]. Exp Cell Res, 2006, 312 (10) : 1831-1842.
  • 4Nilsson J, Yekezare M, Minshull J, et al. The APC/C maintains the spindle assembly checkpoint by targeting Cdc20 for destruction[J]. Nat Cell Biol, 2008,10 (12) : 1411-1420.
  • 5Zhang J,Ahmad S,Mao Y. BubR1 and APC/EB1 cooperate to maintain metaphase chromosome alignment [J]. J Cell Biol, 2007,178(5) : 773-784.
  • 6Dai W, Wang Q, Liu T, et al. Slippage of mitotic arrest and enhanced tumor development in mice with BubR1 haploinsufficiency[ J ]. Cancer Res, 2004,64 (2) : 440-445.
  • 7Rao CV, Yang YM, Swamy MV, et al. Colonic tumorigenesis in BubRiW/-ApcMin/+ compound mutant mice is linked to premature separation of sister chromatids and enhanced genomic instability[J]. Proc Natl Acad Sci USA, 2005, 102 (12) : 4365-4370.
  • 8Klebig C,Korinth D, Meraldi P. Bubl regulates chromosome segregation in a kinetochore-independent manner[J]. J Cell Biol, 2009,185 (5) : 841-858.
  • 9傅云峰,谢幸,叶大风.肿瘤与纺锤体检查点的研究现状[J].国际肿瘤学杂志,2005,32(1):3-6. 被引量：8
  • 10Yu H,Tang Z. Bubl multitasking in mitosis[J]. Cell Cycle, 2005,4 (2) : 262-265.

二级参考文献27

  • 1Sudakin V,Chan GK,Yen TJ.Checkpoint inhibition of the APC/C in HeLa cells is mediated by a complex of BUBR1, BUB3, CDC20, and MAD2. The Journal of Cell Biology . 2001
  • 2Minhas KM,Singh B,Jiang WW,et al.Spindle assembly checkpoint defects and chromosomal instability in head and neck squamous cell carcinoma. International Journal of Cancer . 2003
  • 3Musio A,Montagna C,Zambroni D,et al.Inhibition of BUB1 results in genomic instability and anchorage-independent growth of normal human fibroblasts. Cancer Research . 2003
  • 4Cahill DP,Lengauer C,Yu J,et al.Mutations of mitotic checkpoint genes in human cancers. Nature . 1998
  • 5Nakagawa H,Yokozaki H,Oue N,et al.No mutations of the Bub1 gene in human gastric and oral cancer cell lines. Oncology Reports . 2002
  • 6Wang X,Jin DY,Wong YC,et al.Correlation of defective mitotic checkpoint with aberrantly reduced expression of MAD2 protein in nasopharyngeal carcinoma cells. Carcinogenesis . 2000
  • 7Percy MJ,Myrie KA,Neeley CK,et al.Expression and mutational analyses of the human MAD2L1 gene in breast cancer cells. Genes Chromosomes and Cancer . 2000
  • 8Wang X,Jin DY,Ng RW,et al.Significance of MAD2expression to mitotic checkpoint control in ovarian cancer cells. Cancer Research . 2002
  • 9Lin SF,Lin PM,Yang MC,et al.Expression of hBUB1 in acute myeloid leukemia. Leukemia and Lymphoma . 2002
  • 10Michel LS,Liberal V,Chatterjee A,et al.MAD2 haplo-insufficiency causes premature anaphase and chromosome instability in mammalian cells. Nature . 2001

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江苏医药
2012年 第24期

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