光肩星天牛对植物源挥发物的触角电位和行为反应

EAG and behavioral responses of Asian longhorn beetle Anoplophora glabripennis(Coleoptera:Cerambycidae) to plant volatiles

为给光肩星天牛引诱剂和驱避剂开发提供基础资料,采用触角电位仪和"Y"型管,测定光肩星天牛成虫对顺-3-烯-1-醇、月桂烯、水芹烯、3-蒈烯、S-α-蒎烯、莰烯、R-α-蒎烯、S-β-蒎烯、柠檬烯、罗勒烯、乙酸乙酯和正己烷等12种植物挥发物5个浓度的EAG和9种挥发物5个浓度的行为反应。结果表明:雌、雄虫触角对12种挥发物均有明显的EAG反应(S-α-蒎烯对雄虫除外),特别是在0.4和2mol.L-1两个浓度;雌、雄虫触角对挥发物的感受强度多存在显著差异(P<0.05或P<0.01)。行为测试结果表明:较高浓度的罗勒烯、月桂烯2种挥发物具有明显的驱避作用(P<0.01)(雌虫对月桂烯无明显行为反应),S-α-蒎烯、S-β-蒎烯、乙酸乙酯、R-α-蒎烯、水芹烯、莰烯等6种挥发物具有明显的引诱作用(P<0.01)(雄虫对S-α-蒎烯、雌虫对莰烯,无明显行为反应);柠檬烯对雌、雄虫均无明显的驱、诱作用。

By using EAG instrument and ＂Y＂ pipe, this study examined the EAG responses of Anoplophora glabripennis adults to 12 kinds of plant volatiles, including cis-3-hexen-1-ol, fl-myrcene, phellandrene, 3-carene, S-a-pinene, camphene, R-a-pinene, S-β-pinene, D-limonene, ocimene, ethyl acetate, and hexane at 5 concentrations, and the behavioral responses of the adults to 9 kinds of the volatiles, aimed to provide basic information for the development of attractants and repellents to A. glabripennsis. The antennae of the female and male adults had obvious EAG responses to 12 kinds of the volatiles （except for the male adults to S-a-pinene） , espe- cially at the concentrations of 0.4 and 2 mol . L-1. There exited significant differences （ P〈0.05 or P〈0.01 ） in the feeling strength of the antennae to the volatiles between female and male adults. The results of behavior test showed that higher concentrations ocimene and β-myrcene had obvious repellent effect （P〈0.01） （except myrcene to the female adults）, S-a-pinene, S-β-pinene, ethyl acetate, R-a-pinene, phellandrene, and camphene had obvious attracting effect （P〈 0.01） （except S-a-pinene to the male adults and camphene to the female adults）, whereas D-limonene had no obvious repellent and attracting effect on the male and female adults.