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镉免疫毒性的β-肾上腺素能受体机制研究 被引量:11

Study on β-adrenoceptors mechanism of cadmium immunotoxicity
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摘要 目的 探讨 β 肾上腺素能受体 (β受体 )途径在镉 (Cd)免疫毒性机制中的地位和作用。方法 运用 β受体阻断剂心得安 (Pro)建立肾上腺素能受体阻断的动物模型 ,观察镉单独及用心得安预处理阻断 β肾上腺素能受体后其对脾淋巴细胞 β受体密度、cAMP含量、DNA损伤程度、T淋巴细胞增殖功能及T淋巴细胞亚群的影响。结果 当单独染镉时 ,脾淋巴细胞 β受体密度在中及高剂量染镉组高于对照组 ;脾淋巴细胞cAMP含量随镉浓度的增加而增高 ,有一定的剂量 反应关系 ,并且高剂量染镉组与对照组相比较有显著性升高 ;各染镉组的T淋巴细胞增殖功能与对照比较有降低 ;DNA损伤与对照组差异有显著性。用心得安阻断 β 受体后染镉 ,可见到心得安对照组 ,心得安 +低镉组及心得安 +中镉组 β受体密度比空白对照组显著升高 ,而在低、中、高镉剂量组 β受体密度与心得安对照组相比差异没有显著性意义 ;cAMP含量、脾淋巴细胞增殖转化及T淋巴细胞亚群变化各组间差异亦没有显著性 (P >0 0 5 ) ;SCGE结果显示 ,DNA损伤程度仅在高剂量染镉组高于对照组。结论 镉的免疫毒性机制中 β Objective To study if the beta adrenoceptors mechanism plays important role in the immunotoxicity of cadmium Methods The beta adrenoceptor density, cellular cyclic adenosine 3′,5′monophosphate(cAMP) levels, and DNA damage of rat spleen lymphocytes were studied Beta antagonist propranolol was used to block the beta adrenoceptor Results In the single Cd administration group: beta adrenoceptor density of spleen lymphocytes in the middle and high Cd concentration groups was higher than that in the non Cd treated control group ( P <0 05), the levels of cAMP increased with the increase of Cd doses, and the result of the high Cd group was higher than that of the control group ( P <0 05) The proliferation function of T lymphocytes was lower in each Cd group than that in the control group ( P <0 05) Single cell gel electrophoresis (SCGE) detected that DNA damage of spleen lymphocytes in each Cd group were more severe than the control ( P < 0 05) Pretreated with Pro group: After blocked with propranolol, beta adrenoceptor density increased in the Pro control group and the density in all Cd treated groups was higher than the control group ( P <0 05), but all Cd treated groups showed no statistical significance than the Pro control group ( P >0 05) The cAMP levels, MTT and the subsets of T lymphocytes showed no statistical significance among all the groups ( P >0 05) The results of SCGE showed that DNA damage was most severe only in the high Cd group than other groups ( P <0 05). Conclusion The results suggest that the nerve internal secretion system plays an important role in the immunotoxicity of cadmium, and the beta adrenoceptor mechanism is important in the immunotoxic mechanism of cadmium
出处 《中华预防医学杂志》 CAS CSCD 北大核心 2000年第3期140-143,共4页 Chinese Journal of Preventive Medicine
基金 国家自然科学基金!( 3 95 70 62 6)
关键词 镉中毒 免疫毒性 Β-肾上腺素能受体 大鼠 Cadmium Receptors, adrenergic, beta Lymphocytes Lymphocyte transformation
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参考文献3

  • 1薛彬 薛彬.T淋巴细胞功能的检测.免疫毒理学实验技术[M].北京:北京医科大学中国协和医科大学联合出版社,1995.19-23.
  • 2黎大明,陈成章,宋宏,余贵英,董胜璋,钟赛贤,赵振新,蔡承铿.镉对免疫系统的影响及与前列腺素的关系[J].中国公共卫生学报,1997,16(4):216-218. 被引量:11
  • 3薛彬,免疫毒理学实验技术,1995年,19页

二级参考文献4

  • 1廉旭,中国公共卫生学报,1994年,13卷,3期,168页
  • 2曹友军,国外医学卫生学分册,1993年,20卷,4期,208页
  • 3黎大明,中华预防医学杂志,1992年,24卷,4期
  • 4黎大明,环境与健康杂志,1990年,7卷,3期,142页

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