丙型肝炎病毒基因分型及核心区序列同源性分析

Genotyping of hepatitis C virus and homology analysis of its core gene sequences isolated from different geological regions

目的 研究不同地区丙型肝炎病毒 (HCV)基因型构成及基因变异。方法 HCV感染者血清 6 4份分别采自中国河南省 (2 5份 )和河北省 (18份 ) ,以及瑞典 (11份 )和德国 (10份 ) ,分别进行HCVRNA和基因型检测 ,对 11株HCV分离株核心区基因序列进行测定 ,并用软件分析各地域HCV核心区基因序列的同源性。结果 从中国两省和欧洲两国分离的HCV基因型构成不同 ,在我国血清标本中发现 1b和 2a亚型 ,在瑞典和德国标本中还发现 1a、2c和 3a亚型。在同一地区分离的同一基因型HCV核心区序列的同源性高于不同地区。结论 HCV基因型的构成与地域有关 。

Objective To study the structure of its genotypes and gene variation of hepatitis C virus (HCV) isolated from different geological regions. Methods Serum specimens were collected from patients with HCV infection of Henan and Hebei Provinces of China, as well as of Sweden and Germany. HCV RNA in their serum was detected by retro transcription polymerase chain reaction (RT PCR) and genotyped. The core gene of 11 HCV isolates were sequenced and their homology was analyzed for different geographic regions with software. Results Structure of HCV genotypes, isolated from two provinces of China, and from Sweden and Germany, was not identical. The homology of their core gene sequences of the same HCV genotype isolated from the same region was higher than that from different regions, and subtypes 1b and 2a were found in serum collected from China and subtypes 1a, 2c and 3a in serum from Sweden and Germany. Conclusion Structure of HCV genotypes associated with their geographic distribution and difference in sequences of the same HCV genotype also associated with their geographic regions.