HtrA1在正常孕妇血清及胎盘组织中的表达及其意义

The significance and expression of high temperature requirement A1 in serum and placenta of normal pregnancy

目的探讨HtrA1(high temperature requirement A1,HtrA1)在正常孕妇血清及胎盘组织中的表达及其意义。方法采用酶联免疫吸附试验法(enzyme-linked immunosorbent assay,ELISA)序贯测定1 812例孕妇早、中、晚孕期血清HtrA1浓度;应用免疫组化SABC法检测正常早孕(15例)、中孕(10例)、晚孕(18例)胎盘组织中HtrA1的表达。结果正常孕妇孕期血清HtrA1浓度分布呈对数正态分布,随着孕周的增加,浓度逐渐升高,孕17～20周达峰值;正常妊娠时,随着孕周增加胎盘组织HtrA1表达逐渐增高(平均灰度值与表达强度成负相关),在早期、中期及晚期妊娠平均灰度值分别为156.28±3.12、153.37±2.54和148.69±2.31,差异有统计学意义(P<0.05)。结论 HtrA1与正常胎盘的发育有关,可能通过不同的作用机制参与不同时期胎盘的形成。

Objective To investigate the expression and significance of high temperature requirement A1 （HtrA1） in serum and placenta of normal pregnancy. Methods We consecutively measured serum HtrA1 level in 1 812 cases of normal pregnancy by enzyme-linked immunosorbent assay （ELISA） method and detected the expression of HtrA1 at different stages of normal placenta （including 15 early-stage pregnancy, 10 midtrimester pregnancy and 18 late-stage pregnancy） by immunohistochemical SABC method. Results Normal serum HtrA1 showed a lognormal distribution; it gradually increased with gestational week and reached the peak value in weeks 17-20. With progression of gestational week, HtrA1 expression in the placenta increased, and there was a negative correlation between the mean grey value and the expression. In the early-stage, mid-stage and late-stage pregnancy, the average gray value was 156.28 ± 3.12, 153.37 ±2.54 and 148.69 ± 2.31, respectively （P〈 0.05）. Conclusion HtrA1 is related to normal placental development and may be involved in the formation of the placenta at different stages with different mechanism.