ITO电极上催化伏安法测定锇联吡啶的研究

Determination of Osmium Bipyridine at ITO Electrode by Catalytic Voltammetry

合成了电子转移介体锇联吡啶Os(bpy)3,采用催化伏安法研究了Os(bpy)3在ITO电极上的电化学行为,并对Os(bpy)3在葡萄糖-葡萄糖氧化酶体系中的催化反应动力学常数进行了测定。Os(bpy)3的均相反应速率常数KS为2.07×10-2cm/s,催化反应中一级反应速率常Kf为1.05×10-3s-1,二级反应速率常数Kmed为1.57×104L/(mol.s)。并采用催化伏安法测定Os(bpy)3的检出限为为0.016μmol/L。因此,Os(bpy)3作为电化学标记物,能够用于生物传感器的构建。

An electron transfer mediator osmium bipyridine was synthesized. The electrochemical behaviors of osmi- um bipyridine at ITO electrode were studied and the catalytic reaction kinetics of it in system of glucose/glucose ox- idase was measured. The homogeneous reaction rate constant （Ks ） is 2.07 ×10^-2cm/s, and the first- order reac- tion rate constant （Kf） and second -order reaction rate constant （Kmed） in catalyzed reaction are 1.05 × 10^-3s-1 and 1.57× 10^4 L/（ mol. s）, respectively. The detection limited is 0. 016 μmol/L. Therefore, as an electrochemical label, osmium bipyridine can be used to construct biosensor for the catalyzed signal amplification.