HPLC法测定广玉兰中小白菊内酯

Determination of Parthenolide in Magnolia Grandiflora L.by the Method of HPLC

建立高效液相色谱法测定广玉兰中小白菊内酯含量的方法.采用ZORBAX SB-C18(4.6 mm×150 mm,5μm)色谱柱,以甲醇(A)―水(B)为流动相,梯度洗脱(0～10 min,10%→48%A;10～20 min,48%→80%A),流速1.0 mL/min,柱温30℃,检测波长210 nm.结果表明,小白菊内酯的线性范围为0.188～2.820μg(r=0.999 9),平均回收率(n=9)为99.3%.证实该方法简便、准确、重复性好,可用于广玉兰中小白菊内酯的含量测定.

To develop a HPLC method for determination of Parthenolide in Magnolia Grandiflora L. , a ZORBAX SB - C18 （4. 6 mm x 150 mm, 5 μm） column was adopted. The mobile phase consists of methanol（A） -water（B） for gradient elution （0-10 min, 10%-48% A; 10-20 min, 48%-80% A） , which was pumped at 1.0 mL/min. The detection wavelength was at 210 nm, and the column temperature was maintained at 30 ℃. The results suggested that the linear ranges was 0. 188-2. 820μg （r =0. 999 9） and the average recovery （n = 9） was 99. 3%. The method is simple, rapid, reliable, and can be used for the determination of Magnolia Grandlora L..