摘要
目的:建立海产品中6种工业染料快速检测方法。方法:样品采用乙腈直接匀浆提取、浓缩,C18色谱柱(250×4.6 mm,5μm)洗脱分离,紫外检测器检测,外标法定量。结果:6种工业染料在0 mg/L～5.0 mg/L范围内线性关系良好,相关系数r>0.999,检测限均达到0.04 mg/kg。回收率范围为78%～99%,相对标准偏差均小于7%。结论:该方法快速、简便、实用,适合基层单位日常工作检测使用。
Objective：To develop a method for rapid determination of 6 industrial dyes in seafoods by HPLC.Methods： Sample was homogenized with acetonitrile and concentrated.The separation of these dyes was carried out on a C18 column with 0.02 mol/L ammonium acetate（0.1% formic acid） ＋ methanol as mobile phase.The quantification was accomplished by external standard method.Results： The calibration curves showed good linearity for 6 analytes between 0 mg/L^5.0 mg/L（r0.999） with detection limits of 0.04 mg/kg.The spiked recoveries in aquatic products samples ranged from 78%~99% with RSDs less than 7%.Conclusion： The method was rapid,convenient and practical,and suitable for determination of these 6 industrial dyes in seafoods in daily work.
出处
《中国卫生检验杂志》
北大核心
2012年第12期2806-2808,2811,共4页
Chinese Journal of Health Laboratory Technology
基金
浙江省公益性技术应用研究计划项目(2010C33152)
