微波消解-氢化物发生原子荧光光谱法测定尿中铋

Determination of bismuth in urine by hydride generation-atomic fluorescence spectrometry after microwave digestion

目的:建立了尿中铋的微波消解-氢化物发生原子荧光光谱分析方法。方法:利用铋(Ⅲ)与硼氢化钾反应生成BiH3,然后用氩气载入电热石英炉中原子化,根据产生的荧光强度对尿中铋进行定量。结果:该法的线性范围为0.2μg/L～50μg/L,检出限为0.2μg/L,,加标回收率为89%～101.2%,相对标准偏差(RSD)为0.9%～1.5%。结论:该方法简便、灵敏度高、重复性及准确度好,适于尿中铋的测定。

Objective：To establish a method for determination of bismuth in urine by atomic fluorescence spectrometry after microwave digestion.Methods： Bismuth（III） reacted with potassium borohydride to form BiH3 which was introduced into electrothermic atomizer to produce fluorescence and the bismuth content was quantified according to the fluorescence intensity.Results： The method was linear in the range of 0.2 μg/L^50 μg/L,the detection limit was 0.2 μg/L,the recovery was 89%~101.2%,with the relative standard deviations（RSDs） of 0.9%~1.5%.Conclusion： The method is simple,sensitive,reproducible and accurate and it is suitable for determination of bismuth in urine.