4株荚膜型流感嗜血杆菌分型情况的比较

Comparison of typing among 4 haemophilus influenzae isolates with capsule

目的:为预防和控制Hi相关疾病提供菌株分型资料。方法:分析比较4株从5岁以下幼儿中分离的荚膜型流感嗜血杆菌(Haemophilus influenzae,Hi)的血清分型、生物分型、脉冲场凝胶电泳(PFGE)和多位点序列分型(MLST)的结果。结果:4株荚膜型Hi中,2株Hib均为生物Ⅰ型;1株Hie为生物Ⅲ型;1株与a、f荚膜型抗血清有交叉凝集的Hia/f为生物Ⅰ型。4株PFGE型的相似度,为48.8%～58.5%,其中,2株Hib菌株之间的相似度仅为48.8%;MLST分型,2株Ⅰ型Hib同为ST-95型,1株生物Ⅲ型的Hie为ST-18型,1株生物Ⅰ型的Hia/f可能为新的ST型,等待提交验证。结论:4株荚膜型Hi彼此间遗传关联度小,提示人群中尚未形成优势荚膜型Hi的聚集。Hi当菌株生物型和血清型相同时,PFGE能较好地区分不同来源的菌株。

Objective：To provide fundamental information about bacterial typing for Hi-associated diseases control.Methods： Serotypes,biotypes,PFGE types and MLST（Multilocus Sequence Typing）types of 4 Haemophilus influenzae capsule strains isolated from children under 5 years old were analyzed.Results： It was found that the 2 Hib isolates were biotypeⅠ,the Hie isolate was biotype Ⅲ,and the Hia/f which was cross reacted with antisera a and f capsule types belonged to biotypeⅠ.The similarity of PFGE pattern among the 4 capsule Hi isolates varied between 48.8%~58.5%,and the two biotype I Hibs only 48.8%.The ST types of 2 Hibs with the same biotype Ⅰwere both ST95,while the biotype Ⅲ Hie was ST-18.The biotype ⅠHia/f maybe a new ST type,waiting for submitting and verification.Conclusion： The association among 4 capsuled Hi is comparatively weak,suggesting thare is no dominant capsuled Hi type among human group.PFGE can be used to identify the source of Hi strains with the same serotype and biotype.