一起由蜡样芽孢杆菌引起的食物中毒的病原学检测

Pathogenic detection of a food poisoning due to contamination with Bacillus cereus

目的:快速分离病原菌,追溯其食源性疾病的来源。方法:采集食物中毒者剩余食物、呕吐物、粪便,进行病原学检测,同时采集病人的双份血清进行相应的抗体检测。结果:在1份凉皮、1份呕吐物、2份大便中检出蜡样芽孢杆菌。其中凉皮、呕吐物中蜡样芽孢杆菌菌落总数>106cfu/g,病人双份血清中,蜡样芽孢杆菌抗体有四倍以上的增高。结论:按照WS/T.82-1996诊断标准,这是一起被蜡样芽孢杆菌污染的凉皮引起的食物中毒。

Objective：To separate pathogenic bacteria quickly and retrospect the source of food-borne illness.Methods： Remaining food,vomits and feces of those food poisoning patients were detected according to etiology characteristics,meanwhile,antibody detection of double serum in patients was done.Results： Bacillus cereus was detected respectively from cold noodle and vomits,with bacteria count 106 cfu/g,and Bacillus cereus was also found in 2 feces samples.Bacillus cereus antibody from patients′ serum increased more than 4 times.Conclusion： According to Diagnosis Standard WS/T.82-1996,this case of food poisoning was caused by cold noodle contaminated with Bacillus cereus.