InvA-PCR快速检测鼠肾标本中钩端螺旋体

InvA-PCR for rapid detection of leptospira in rat kidney specimens

目的:探讨InvA-PCR方法快速检测钩端螺旋体的应用价值。方法:对2009年淳安县106只鼠肾标本直接提取其DNA用invA-PCR法进行检测,同时对106只鼠肾标本进行分离培养和显微镜凝集试验(MAT)血清学鉴定。结果:106只鼠肾标本经InvA-PCR法检测,9份标本钩端螺旋体invA基因呈阳性,阳性率为8.5%。106只鼠肾标本分离培养,分离到3株钩端螺旋体菌株,均为黄疸出血群,阳性率为2.8%。3份培养阳性标本InvA-PCR均阳性。结论:InvA-PCR方法操作方便、耗时短、特异性强、灵敏度高,可作为致病性钩端螺旋体可靠的快速诊断方法,可用于钩体病的流行病学调查和钩体病临床标本快速初筛。

Objective：To validate an InvA - PCR method for rapid detection of leptospira in rat kidney speci- mens. Methods：The InvA-PCR method was used to detect leptospira in 106 rat kidney specimens collected from Chun＇an county in 2009. At the same time, bacterial culture was applied to the specimens, and microscopic aggluti- nation test （MAT） was employed for serological identification when positive culture was gotten. Results：Out of 106 rat kidney specimens, 9 cases （8.5%） were leptospiral invA gene positive by the InvA - PCR, while 3 （2.8%） strains of Leptospira were isolated. All of these 3 isolates belonged to the serogroup of icterohaemorrhagiae. The 3 specimens with positive culture were all positive by the InvA - PCR assay. Conclusion： InvA - PCR method has the advantages of convenient operation, short time consumption, strong specificity and high sensitivity. It can be used as a reliable rapid diagnostic method for pathogenic Leptospira in leptospirosis epidemiology investigation and leptospiro- sis quick screening in clinic.