苦参碱诱导宫颈癌Hela细胞的凋亡作用

Effects of matrine on apoptosis hnduction in Cervi Calcarcinoma hela cells

目的:研究不同浓度的苦参碱(Mat)对宫颈癌Hela细胞株的体外增殖抑制作用。方法:采用体外细胞培养技术,以不同浓度的Mat作用于宫颈癌Hela细胞,相同条件下培养24h、48h、72h后,倒置显微镜观察细胞形态变化;MTT法测定Mat对Hela细胞的增殖抑制率;流式细胞仪(FCM)检测细胞凋亡率。结果:不同浓度Mat在体外均能不同程度抑制宫颈癌Hela细胞增殖,并且作用呈现时间-剂量依赖性;苦参碱诱导Hela细胞凋亡率增加。结论:Mat有抑制宫颈癌Hela细胞的增殖,诱导其凋亡作用。

Objective： To study the inhibitory effects of matrine at different concentrations on cervical carcinoma Hela cells in vitro. Methods：Matrine at different concentrations （0. 5,1.0,1.5 and 2.0mg/ml） was re spectively used to cultivate cervical cancer Hela cells in vitro. After Hela cells were cultivated for 24, 48 and 72 hours respectively, the proliferation of Hela cells treated with matrine was observed by discrepancy microscope; the effect of matrine on cervical cancer cells was assayed by MTT and flowcytometry（FCM）. Results： Matrine of differ ent concentration could significantly inhibit proliferation of Hela cells, and its inhibitory effects increased as the con centration higher. It was shown that the inhibitory efficacy of matrine in vitro depended on the reaction time and dos- age. The effect on apoptosis rate was also increased. Conclusions： Matrine can significantly inhibit proliferation of cervical carcinoma Hela cells, and induce apoptosis of them.