微量元素亚硒酸钠与硫酸镍对Epstein—Barr病毒壳抗原表达影响的研究

STUDIES ON THE INFLUENCE OF SODIUM SELENITE AND NICKEL SULFATE UPON THE CAPSID ANTIGEN EXPRESSION OF EPSTEIN-BARR VIRUS

本文报告应用间接免疫荧光技术,研究了亚硒酸钠与硫酸镍对EB病毒壳抗原(VCA)表达的影响及其相互关系,0.1-1.0μg/ml亚硒酸钠1-20μg/ml硫酸镍,各自对VCA表达呈抑制和促进的相反效应。适当浓度的亚硒酸钠不仅能抵消各剂量硫酸镍对病毒抗原表达的促进作用,而且还明显抑制抗原自身的表达。时经镍预处理24小时后的靶细胞,硒仍显示出强烈的抑制效应:外一方面,硒一次预处理后,仍保持其对镍激发抗原能力的抑制。这些结果提示,硒对EBV-VCA表达的抑制作用远较镍的促进作用为强,而且持久。 本文还讨论了鼻咽癌患者体内的高镍低硒状态与EBV感染的相互关系及其可能在鼻咽癌发生、发展中所起的作用。

Influences and relationship between sodium selenite and nickel sulfate onVCA expression of EBV were studied. B95-8 cells were used as target cells and the VCA positive cells were evaluated by indirect immunofluorescence staining. Non-toxic doses of Na2SeO3 (0.1-1.0 *g/ml) and NiSO4 ? 6H2O(1-20*g/ml)exnibited inversive effect of inhibition and promotion respectively on the antigen expression. The promotion effect of NiSO4 on VCA expression, as well as the natural expression of VCA, could be effectively inhibited by the simultaneous presence of selenium in the medium. The inhibitory effect was also seen with the cells either 24 hours pretreated or post-treated by NiSO4.