脂血对Sysmex XE-2100D血液分析仪检测指标的影响研究

The research on the influence of lipemia on the detection parameters of Sysmex XE-2100D hematology analyzer

目的探讨脂血对Sysmex XE-2100D血液分析仪测定血常规主要指标的影响。方法取20例无溶血、黄疸、脂血标本设为对照组,将这20例标本去除上层血浆成分,加入同体积的Sysmex XE-2100D血液分析仪原装配套稀释液,测定血常规设为实验组,将其结果与对照组进行比较;取66例无溶血、黄疸、脂血已测定的血常规标本,随机分为3组,用实验组相同的方法去除10、50和100μL血浆后,加入等体积脂肪乳剂,用日立7600全自动生化分析仪测定甘油三酯(TG),根据TG的含量不同,将脂血的程度进行分级,TG 6.0～10.0 mmol/L 21例,设为轻度脂血组,未加脂肪乳剂测定结果设为对照组A;TG 10.1～14.0 mmol/L 21例,设为中度脂血组,未加脂肪乳剂测定结果设为对照组B;TG﹥14.0 mmol/L 24例,设为重度脂血组,未加脂肪乳剂测定结果设为对照组C,将测定结果分别与其未加脂肪乳剂者的测定结果进行比较。结果对照组A与轻度脂血组比较13项参数结果差异均无统计学意义;中度脂血组与对照组B和重度脂血组与对照组C比较,血红蛋白(Hb)、平均红细胞血红蛋白含量(MCH)、平均红细胞血红蛋白浓度(MCHC)、白细胞(WBC)均升高,其4项指标差异有统计学意义(P<0.05)。结论中、重度脂血对Sysmex XE-2100D血液分析仪检测血液常规Hb、MCH、MCHC、WBC参数影响较大,并随着脂血程度加深而加重。

Objective To investigate the influence of lipemia on the main parameters of Sysmex XE-2100D hematology analyzer. Methods A total of 20 samples without hemolysis, jaundice and lipemia were set as control group. The upper plasma was removed, and equal volume reagent diluent of Sysmex XE-2100D hematology analyzer was added. These samples were performed as experiment group, and the results were compared with those of the control group. A total of 66 routine blood samples without hemolysis, jaundice and lipemia were randomly classified into 3 groups. These samples were treated by removing 10,50 and 100 μL plasma and adding equal volume of fat emulsion, and then the levels of triglyceride （TG） were determined by Hitachi 7600 automatic biochemistry analyzer. Lipemia level was classified based on TG level. A total of 21 samples （ TG 6.0- 10.0 mmol/L） were set as low lipemia group, and original samples without fat emulsion were set as control A. A total of 21 samples （TG 10.1 -14.0 mmol/L） were set as moderate lipemia group, and original samples without fat emulsion were set as control B. A total of 24 samples （TG 〉 14.0 mmol/L） were set as high lipemia group,and original samples without fat emulsion were set as control C. The detection results of different lipemia level groups were compared with the results of control groups. Results In the low lipemia group, the main parameters of blood routine test showed no statistical significance comparing to control A. Comparing to control B and C, hemoglobin （Hb）, mean cell hemoglobin （MCH）, mean corpuscular hemoglobin concentration （MCHC） and white blood cell （WBC） increased significantly in the moderate and high lipemia groups, respectively （P 〈 0.05 ）. Conclusions The moderate and high lipemia levels of blood can influence significantly the results of blood routine test by Sysmex XE-2100D hematology analyzer. Hb, MCH, MCHC and WBC increase parallelly with the increase of lipemia level.