导流杂交技术检测中原地区女性人乳头瘤病毒感染情况

Detection of human papillomavirus infection by channelization hybridization among Chinese women in central plains

目的研究中原地区人乳头瘤病毒(HPV)感染率及亚型分布,探讨HPV感染与年龄的关系。方法采用聚合酶链反应(PCR)及导流杂交技术对11 861例就诊的女性进行HPV分型检测。结果 HPV感染率为29.0%,其中高危型HPV16感染率最高(8.3%),其次依次为低危型HPV6(6.0%)、低危型HPV11(5.5%)、高危型HPV52(4.6%)、高危型HPV58(3.8%)。不同年龄组间HPV感染率差异有统计学意义(P<0.01),20～30岁组感染率(42.8%)最高,其次为30～40岁组(25.7%)和50岁以上组(23.2%),最低的为40～50岁组(17.9%)。结论中原地区女性HPV感染的优势亚型为HPV16,20～30岁女性HPV感染率最高。

Objective To investigate the infection rate of human papillomavirus （ HPV）, the distribution of HPV subtypes and the relationship between HPV infection and age. Methods Polymerase chain reaction （PCR） and channelization hybridization were used to detect the subtypes of HPV among 11 861 women. Results The infection rate of HPV was 29.0%. The predominant subtype was high risk-HPV16 （8.3%） , followed by low risk-HPV6 （6.0%）, low risk-HPv11 （5.5%）, high risk-HPV52 （4.6%） and high risk-HPv58 （3.8%）. The infection rate of HPv was statistically significant in different age groups （P 〈 0.01 ）. The highest infection rate distributed in 20-30 years old group （42.8%）, followed by 30- 40 years old group （25.7%） , more than 50 years old group （23.2%） and 40-50 years old group （ 17.9% ）. Conclusions The predominant subtype of HPV infection is HPV16, and the 20-30 years old women have the highest infection rate in Chinese central plains.