分支链DNA Alu技术定量检测败血症患者血浆循环DNA的表达

bDNA-based Alu quantitative assay of plasma cell-free DNA level in sepsis patients

目的探讨血浆循环DNA(cf-DNA)水平定量检测在败血症诊断和败血症与全身炎症反应综合征(SIRS)鉴别诊断中的价值。方法利用分支链DNA Alu技术定量检测24例败血症患者、43例SIRS患者和73名健康人血浆cf-DNA的表达水平;绘制受试者工作特征(ROC)曲线评估血浆cf-DNA的临床价值。结果正常对照组血浆cf-DNA表达水平为68.56(12.56～309.78)ng/mL,SIRS组为609.67(15.98～2 596.87)ng/mL,败血症组为1 398.96(19.28～2 987.56)ng/mL,败血症组明显高于正常对照组(P<0.001)和SIRS组(P<0.001)。SIRS组与正常对照组、败血症组与正常对照组、败血症组与SIRS组的曲线下面积(AUC)分别为0.763(0.661～0.865)、0.955(0.884～1.025)和0.777(0.663～0.891)。结论血浆cf-DNA具有作为新的败血症诊断标志物的临床应用价值。

Objective To investigate the significance of plasma cell-fee DNA （cf-DNA） in diagnosis ot sepss and differential diagnosis between sepsis and systemic inflammatory response syndrome （SIRS）. Methods The plasma cfDNA of 24 sepsis patients, 43 SIRS patients and 73 healthy controls were detected by bDNA-based Alu quantitative assay. Receiver operating characteristic （ROC） curve was performed to evaluate the clinical significance of plasma cf- DNA. Results The plasma ef-DNA significantly increased in sepsis patients compared with in SIRS patients and healthy controls [ 1 398.96 （ 19.28-2 987.56） ng/mL, 609.67 （ 15.98-2 596.87 ） ng/mL and 68.56 （ 12.56-309.78 ） ng/mL, respectively, P 〈 0. 001 ]. The areas under the ROC curve （AUC） of cf-DNA for the SIRS patients with healthy controls, sepsis patients with healthy controls and sepsis patients with SIRS patients were 0. 763 （0. 661-0. 865 ） , 0. 955 （0. 884-1. 025） and 0. 777 （0. 663-0. 891 ）, respectively. Conclusions Plasma cf-DNA has a clinical significance as a new biomarker for the diagnosis of sepsis.