海口市273例体检人群阴道中假丝酵母菌种类的初步研究

Preliminary study of distribution of Candida species in the vulvovaginal of women for health checkup in Haikou

目的研究妇科体检人群中阴道假丝酵母菌的分布情况。方法将收集的273例妇检阴道拭子保存在含有抗生素的YEPD液体培养基中,然后接种到YEPD平板上分离培养。分离出的酵母菌通过菌落形态、制片革兰染色,乳酸酚棉蓝染色及显色培养基作初步鉴定;并通过芽管形成试验、厚膜孢子、假菌丝生成试验及DNA ITS序列分析进行菌种鉴定。结果 273例标本中分离出47株(17.2%)假丝酵母菌,其中白假丝酵母菌24株(51.1%),光滑假丝酵母菌13株(27.7%),克柔假丝酵母菌8株(17.0%),热带假丝酵母菌1株(2.1%),其他假丝酵母菌1株(2.1%)。进一步分析显示,不同年龄段和不同妇科疾病的假丝酵母菌检出率差异均无统计学意义(P>0.05)。结论体检人群外阴阴道中的假丝酵母菌致病菌种类以白假丝酵母菌为主,其次是光滑假丝酵母菌。假丝酵母菌感染与年龄、妇科疾病种类的关系不明显。

Objective To study the distribution of Candida species in the vulvovaginal of women for health checkup.Methods The 273 vaginal swabs were kept in YEPD liquid medium containing antibiotics,and then transferred onto YEPD Agar for culture and isolation of yeasts.Preliminary identification of the isolated yeasts was performed by colony morphology,Gram stain,lactic acid phenol cotton blue stain and chromogenic medium,and strain identification was tested by germ tube,chlamydospore test and pseudohypha production test and DNA ITS sequence analysis.Results There were 47 Candida stains（17.2%） in 273 specimens,including 24 C.albicans（59.1%）,13 C.glabrata（27.7%）,8 C.Kmsei（17.0%）,and 1 C.tropicalis（2.1%） and other Candida strains（2.1%）.There were no statistical significance in infection rate of Candida among different age groups and different gynecologic diseases（P0.05）.Conclusion C.albicans is the main strain of vulvovaginal pathogenic bacteria in women for health checkup.,followed by C.glabrata.No obvious relationships between Candida infection and age or gynecologic diseases were observed.