纤溶活性化合物GDG对溶菌酶结晶特性的影响

Crystallization of Lysozyme by GDG as a Fibrinolytic Compound

鸡蛋白溶菌酶的稳定性、溶解性和易结晶性,被结晶学家作为蛋白质结晶理论的模板而深入研究。通过优化四方晶系溶菌酶的结晶条件,在含5%氯化钠和0.02%叠氮化钠,pH5.2的0.1mol/L醋酸钠缓冲溶液中,15mg/mL溶菌酶晶核数量较少,晶体尺寸最佳。向溶菌酶中添加GDG,得到添加GDG(CGDG/CHEWL分别为2:1,1:1,1:2)的溶菌酶晶体,并对添该晶体进行同步辐射光源衍射,收集其三维结构数据。结果显示GDG可促进溶菌酶晶核的形成,但晶体的尺寸有所减小。由电子密度图可知GDG对溶菌酶的主链和氨基酸残基侧链没有影响。

Lysozyme was extensively studied as a model of protein crystallization,we identified that 15 mg/mL lysozyme,0.1 mol/L sodium acetate buffer containing 5% sodium chloride and 0.02% sodium azide,pH 5.2,crystal nucleus were less and crystals could reach a certain size.After adding GDG to lysozyme,we obtained the crystal of lysozyme added GDG,CGDG/CHEWL=2：1,1：1,1：2,studied the effect of GDG on crystallization of lysozyme and performed the synchrotron radiation diffusion and collected the three dimensional structure data of lysozyme added GDG.It is shown that increased in number of crystal amount and decreased in volume of crystal size in crystallization of lysozyme accompanied by the concentration of GDG.It was not displayed effect on the main chain and most side chains of lysozyme in the presence of GDG in the electron density map.