勋章菊试管开花研究

Study of Flowering in Vitro of Gazania rigens L.

试管花卉有较高的观赏和科研价值,勋章菊适宜作为试管花卉的材料进行研究和推广。以勋章菊叶片诱导的不定芽为材料,通过不同的培养基处理进行开花诱导,探讨勋章菊在试管内开花的条件。研究结果表明:多效唑和矮壮素对勋章菊的花芽分化有促进作用,而且使植株矮化、增壮,提高了观赏价值。MS+0.5mg/L 6-BA+0.1 mg/L NAA+0.4 mg/L PP333和MS+0.5 mg/L 6-BA+0.1 mg/L NAA+0.7 mg/L CCC的处理最好,60 d花芽诱导率分别为54.4%和50.2%。

Vitro flower has higher ornamental and scientific research value.Gazania rigens L.is one kind of perfect vitro plants for research and promotion.In the experiment,the adventitious buds of Gazania rigens L.were used as the material through different medium of flowering induction to study flowering conditions of Gazania rigens L.in vitro.The results showed that PP333 and CCC can promot flower bud differentiation of plant,and made the plant short and strong.Thus the ornamental value of Gazania rigens L is improved.The best media are MS+0.5 mg/L 6-BA+0.1 mg/L NAA+0.4 mg/L PP333 and MS+0.5 mg/L 6-BA+0.1 mg/L NAA+0.7 mg/L CCC.The induction rates of flower bud are 54.4% and 50.2% respectively.