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Expression Analysis of the ANS Gene in Fragaria x ananassa cv, Toyonaka

Expression Analysis of the ANS Gene in Fragaria x ananassa cv, Toyonaka
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摘要 Strawberry is an economically valuable crop all over the word. Its fruits contain large amounts of polyphenol compounds, anthocyanins for instance. Anthocyanins play important roles in attracting pollinators and protecting plants from various stresses. ANS (Anthocyanidin synthase) catalyzes the conversion of leucoanthocyanidins to colored anthocyanidins. In this study, the total anthocyanins concentration at seven developmental stages of the strawberry fruit were investigated by UV spectrophotometry at 496 nm and 700 rim; and the expression levels of the Fa-ANS gene were studied by qRT-PCR. The results showed that the anthocyanins contents gradually increased along with the fruit maturation; while the expression patterns of Fa-ANS were consistent with the anthocyanins.
出处 《Journal of Life Sciences》 2012年第11期1185-1189,共5页 生命科学(英文版)
关键词 Anthocyanins ANS strawberry fruit developmental stages. 草莓果实 NS基因 表达分析 花青素含量 定量RT-PCR 紫外分光光度法 品种 多酚类物质
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