右旋糖酐酶降解小分子右旋糖酐制备特微分子右旋糖酐

Preparation of Super Micro-molecular Dextran by Degradation of Small Molecule Dextran with Dextran Enzyme

右旋糖酐酶是降解右旋糖酐的专一的酶，用右旋糖酐酶可将分子量较大的右旋糖酐20（重均分子量为16000～24｜D00Da）降解为分子量较小的特微分子右旋糖酐（重均分子量则为3000～6000Da）。试验结果表明，以右旋糖酐20为原料，按底物浓度为30％（Ⅵ唧），反应时间为10min，酶用量为69U·g-1的生产工艺条件生产，得到的特微分子右旋糖酐产物的质量稳定，分布宽度指数小，分子量分布范围窄。

The dextran enzyme was a special enzyme for degradation of dextran. It could degrade dextran-20 （average Mw 16000-24000Da） to super micro-molecular dextran （average Mw 3000-6000Da）.The results showed that when the substrate concentration was 30%（w/w）, the reaction time was 10min and enzyme dosage was 69U/g, Dextran-20 was degraded to super micro- molecular dextran. The product had excellent performance, stable quality and narrow molecule weight distribution.