鼓泡式生物反应器培养人参不定根的研究

Cultivation of Panax ginseng adventitious roots in bubble bioreactors

目的:研究鼓泡式生物反应器对人参不定根培养的影响。方法:利用组织培养技术结合高效液相色谱法和紫外分光光度法,考察不同角度的鼓泡式生物反应器对人参不定根生长及人参不定根有效成分皂苷和多糖合成的影响。结果:不同生物反应器培养人参不定根中,适合培养人参不定根生长的最佳反应器类型为120°圆锥型鼓泡式生物反应器。测定120°圆锥型反应器内的人参不定根生长呈典型的"S"型曲线。人参不定根在第40天得到最大的生物量,并且鲜重、干重、干重增殖倍数都达到了最大值,分别为113.15 g,9.62 g,63.13倍。人参不定根总皂苷和多糖质量分数为2.25 mg.g-1和2.73%。结论:适合培养人参不定根的最佳反应器为120°圆锥型鼓泡式生物反应器。该实验为工业化生产人参有效活性成分奠定基础。

Objective： To study cultivation of Panax ginseng adventitious roots in bubble bioreactors. Method： The adventitious roots were obtained through tissue culture different types of bioreactors. The contents of ginsenosides Re, Rbland Rg1 were deter- mined by HPLC while the contents of polysaccharides were determined by ultraviolet spectrophotometry. Result： The results showed that of the three types tested, the most efficient bioreactor for cultivation of the ginseng adventitious roots was the cone-type bioreactors （ with the 120° ） , in which, the growth curve of adventitious roots was S-shaped. The maximum biomass was obtained on the 40th day, with the fresh weight, dry weight and growth rate reaching the maximum, which were 113.15 g, 9.62 g and 63.13 times respectively, and the concomitant contents of polysaccharide and ginsenoside were 2. 73 % and 2. 25 mg · g^-1. Conclusion： The results showed that the most efficient bioreactor for cultivation of the ginseng adventitious roots was the cone-type bioreactors（ with the 120°）. These results provide a theoretical reference for developing an efficient production process of active metabolites of ginseng in the scale-up cultivation.