“芪香”气雾剂对流感病毒肺炎小鼠肺部炎性损伤的影响及机制研究

Study on Effect and Mechanism of Qixiang Aerosol on Lung Inflammatory Injury of Mice with Influenzal Pneumonia

目的:探讨"芪香"气雾剂对流感病毒肺炎小鼠肺部炎性损伤的影响及机制。方法:以流感病毒亚甲型鼠肺适应株A/PR/8/34(H1N1)感染小鼠为模型,用常规HE染色法观察"芪香"气雾剂对肺部炎性损伤的影响;采用ELISA法观察血清IFN-γ、IL-10含量。结果:"芪香"气雾剂预防给药后,与模型组比较,肺部病变明显减轻,有效控制炎症反应的程度和范围,阻止病灶的蔓延,显著减轻小鼠肺部炎性损害;提高血清IFN-γ、IL-10含量。结论 ":芪香"气雾剂可减少流感引起的肺部炎性损伤,其机制可能与调节促炎因子IFN-γ与抗炎因子IL-10相对平衡的状态有关。

Objective：To investigate effect and mechanism of Qixiang Aerosol on lung inflammatory injury of mice with influenzal pneumonia.Methods：The mice were infected with A/PR/8/34（H1N1）.The effect of Qixiang Aerosol on lung inflammatory injury was observed by conventional HE staining method,and contents of IFN-γ and IL-10 in blood serum were tested by ELISA.Results：After prophylactic medication of Qixiang Aerosol,compared with model group,the pathological changes of lung were relieved significantly,the severity and range of inflammation were controlled effectively,the spread of lesions was prevented,the lung inflammatory injury was relieved obviously,and the contents of IFN-γ and IL-10 were increased.Conclusion：Qixiang Aerosol can relieve the lung inflammatory injury caused by influenza,and the mechanism may be related with the relative balance between IFN-γ which is the regulating proinflammatory cytokines and IL-10 which is the anti-inflammatary factor.