苦豆子总碱对大鼠膀胱逼尿肌作用机制研究

Contraction Effects of Total Alkali Sophora Alopecuroids on Rat's Bladder Detrusor Muscle Strips in Vitro

目的:观察苦豆子总碱(total alkali sophora alopecuroids L,TASa)对大鼠膀胱离体肌条的作用及机制。方法:采用恒温灌流浴槽,记录膀胱逼尿肌收缩波的振幅、持续时间、收缩面积和频率,给予TASa(5、10、20、40、80 mg/L)观察其量效关系(r);将标本分为正常组(NS)、TASa、TASa+异搏定(10-7mg/L)、TASa+酚妥拉明(10-6mg/L)、TASa+苯海拉明(10-6mg/L)、TASa+消炎痛(10-6mg/L)和TASa+阿托品(10-6mg/L)七组探讨TASa兴奋逼尿肌的作用机制。结果:TASa能增强逼尿肌收缩的振幅、持续时间和收缩面积,并有量效依赖性(振幅r=0.95,t=5.43,P<0.05;持续时间:r=0.97,t=7.99,P<0.01;收缩面积:r=0.97,t=7.03,P<0.01),从TASa(20 mg/L)浓度开始可加快其收缩频率(P<0.01)。异搏定可抑制TASa兴奋逼尿肌的作用,而阿托品、苯海拉明、消炎痛和酚妥拉明则无影响。结论:TASa可能是激活Ca2+通道,升高胞浆内Ca2+触发逼尿肌的收缩。

Objective：To study the contraction effects of the total alkali of Sophora alopecuroids L（TASa）on rat＇s bladder detrusor muscle strips in vitro and the possible mechanisms.Method：The constant temperature perfusion of the bath machine was used to record amplitude,duration,contraction area and frequency of the contraction bladder detrusor muscle in vitro.TASa was divided into five doses groups（5,10,20,40,80mg/L）,giving TASa with different doses to bladder detrusor muscle in vitro and the dose-effect relationship between muscle contraction and different doses of TASa was observed.Bladder detrusor muscle was divided into seven groups,including control group,TASa group,TASa＋10-7mg/L verapamil group,TASa＋10-6mg/L phentolamine group,TASa＋10-6mg/L benzhydramine group,TASa＋10-6mg/L indomethacin group,TASa＋10-6mg/L atropine group.The contraction effects of TASa were investigated.Results：TASa can increase bladder detrusor muscle contraction amplitude,contraction duration,contraction area,and has an obvious concentration-dependent contraction（contraction amplitude r=0.95,t=5.43,P0.05;contraction duration r=0.97,t=7.99,P0.01;contraction area r=0.97,t=7.03,P0.01）,and TASa（20mg/L）begains to accelerate the contraction frequency of bladder detrusor muscle.Verapamil can inhibit the bladder detrusor muscle contraction of TASa,however atropine,benzhydramine,indomethacin and phetolamine have no contraction effect.Conclusion：TASa may activate calcium channel,increase calcium flow to the interior of the cell and increase bladder detrusor muscle＇s contraction.