北豆根中蝙蝠葛碱在小鼠体内的组织分布研究

Distribution of Dauricine.Dau in Mice Tissue in Rhizoma Menispermi

目的:采用小鼠尾静脉注射和灌胃给药的方法考察北豆根中蝙蝠葛碱在小鼠体内的分布特征。方法:小鼠组织匀浆液经沉淀蛋白,以缓冲液(0.68mL磷酸和0.71mL的三乙胺加纯净水至500mL,过0.22μm水系微孔滤膜)-乙腈(76∶24)为流动相,经Hypersil ODS2(4.6mm×150mm,5μm)分离,检测波长为281nm,进行色谱分析。结果:小鼠经静脉注射15mg/kg和灌胃100mg/kg给药后,组织分布结果显示,两种方法给药后各脏器组织的药物含量均明显高于血浆药物浓度,灌胃给药后,药物含量依次为胃、肠、肝、脾、肺、肾、心、肌肉、脑、血浆。结论:蝙蝠葛碱在小鼠体内的分布相对广泛,各脏器均有分布且药量均明显高于血浆药物浓度,且在动物体内有一定的蓄积作用。

Objective：To study on the characteristics of distribution of Dauricine.Dau in mice in vivo in Rhizoma Menispermi by i.v.and i.g..Methods：After the tissue homogenate was precipitated,the analysis was conducted on a ODS-C18（Hypersil ODS2,4.6mm×150mm,5μm）column with mobile phase consisting of acetonitrile： Phosphoric Acid solution（76∶24）.The detective wavelength was 281 nm.Result：The results of experiment of distribution of Dau in mice after i.v.（15mg/kg）and i.g.（100mg/kg） showed that all organs had distribution and the charge of Dau in tissues was significantly higher than that in plasma.Conclusion：The distribution of Dauricine in animal in vivo relatively wide,and the drugs in animal body has a certain accumulation.