树舌多糖GF抑制肝癌细胞SMMC7721增殖及对CDK2基因表达的影响

Effects of GAPSGF on Inhibiting Hepatoma Cell Line SMMC7721 Proliferation and CDK2 Expression

目的:探明树舌多糖GF(Ganoderma appanatum polysaccharides GF,GAPSGF)对肝癌细胞SMMC7721增殖及CDK2表达的影响。方法:不同浓度树舌多糖GF处理肝癌细胞SMMC7721,采用MTT法检测树舌多糖GF对肝癌细胞SMMC7721增殖抑制作用;实时定量聚合酶链反应(Real-TimePCR)检测CDK2 mRNA表达;蛋白印迹法(Western blot)检测CDK2蛋白表达情况。结果:树舌多糖GF对肝癌细胞SMMC7721具有明显的生长抑制作用。其中树舌多糖GF2.5μg/mL组与树舌多糖GF10μg/mL组作用显著,且CDK2的mRNA和蛋白表达下降。结论:树舌多糖GF可能通过降低CDK2表达,抑制肝癌细胞SMMC7721增殖。

Objective： To study the effects of Ganoderma appanatum polysaccharides GF（GAPSGF）on proliferation and CDK2 expression in hepatoma cell line SMMC7721.Methods： MTT assay was used to examine the growth and proliferation of SMMC7721 cells after treatment with different concentrations of GAPSGF.Real-time PCR and Western blot were performed to measure the expression of CDK2 at mRNA and protein level.Results： GAPSGF could effectively inhibit proliferation of hepatoma cell line SMMC7721,and GAPSGF at concentration of 2.5μg·mL-1 and 10μg·mL-1 had a significant effect on inhibiting proliferation.The expression of mRNA and protein of CDK2 effectively reduced.Conclusion： GAPSGF restrains proliferation of hepatoma cell line SMMC7721 by reducing CDK2 expression.