超强静磁场照射对小鼠抗氧化和免疫能力的影响

Effects of Exposure to Ultra High Static Magnetic Fields on Antioxidation and Immune Functions of Mice

研究超强静磁场(USMF)连续照射对小鼠抗氧化和免疫能力的影响.采用12 T的超强静磁场每天照射小鼠2 h,连续7 d,测量小鼠肝脏和肾脏中丙二醛(MDA)的含量和过氧化氢酶(CAT)、超氧化物歧化酶(SOD)、总抗氧化能力(T-AOC)和谷胱甘肽过氧化物酶(GSH-Px)的活力;测定胸腺、脾脏指数和脾脏中淋巴细胞转化率;检测外周血中白细胞数目.结果表明:超强静磁场可以显著增强肝脏中SOD和GSH-Px的活力以及肾脏中CAT,SOD,GSH-Px和总抗氧化能力;降低肝脏和肾脏中MDA含量;明显提高小鼠脾细胞淋巴转化率、胸腺指数和外周血中白细胞数目.超强静磁场对小鼠连续照射可以提高小鼠机体的抗氧化和免疫能力.

Study the effects of exposure to ultra high static magnetic fields on antioxidation and immune functions of mice. Four-week old mice were exposed to the static magnetic fields with magnetic intensity of 12 Tcsla （T） for 2 hours every day, and continued for 7 days. On the ninth day, the mice were sacrificed. Livers and kidneys were taken to measure the activity of Catalase （CAT）, Superoxide Dismutase （ SOD ）, Total antioxidant capacity （ T-AOC ）, Glutathione peroxidase （GSH-Px） and the content of Maleic Dialdehyde （MDA）. Also, thymus and spleen were obtained to measure their index and the splenic lymphocyte proliferation. Besides, blood of mice was collected by taking the eyeball and the content of white cells in the peripheral blood of mice was detected. Compared to control group, activity of CAT, SOD, T-AOC and GSH-Px in kidney in the experiment group were increased significantly. The content of MDA was decreased significantly. Activity of SOD and GSH-Px in liver was enhanced, and the content of MDA decreased significantly. Furthermore, splenic lymphocyte proliferation was increased, and the index of thymus and the content of white cells in peripheral blood were increased remarkably. Exposure to ultra high static magnetic fields can improve antioxidation and immune functions of mice.